Description

9-(3'-Azido-3'-Deoxy-β-D-Xylofuranosyl)Adenine 5'-Triphosphate is a specialized nucleoside triphosphate analog, a critical building block for advanced biochemical research and development. This compound is engineered to serve as a chain terminator and labeling agent in nucleic acid sequencing and synthesis applications. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in molecular biology, enzymology, and the development of novel therapeutic agents.

Application

• Next-Generation Sequencing (NGS): Acts as a reversible terminator in sequencing-by-synthesis platforms for high-throughput genomic analysis.
• Enzymatic Synthesis: Used as a modified substrate for DNA/RNA polymerases to study enzyme kinetics and incorporate specific functionalities.
• Probe Labeling: Enables the site-specific incorporation of azide groups into oligonucleotides for subsequent bioorthogonal conjugation (e.g., Click Chemistry) with fluorescent dyes or other reporter molecules.
• Structural Biology Studies: A tool for investigating the active sites and mechanisms of nucleic acid-processing enzymes through X-ray crystallography or NMR.
• Antiviral Research: Serves as a potential nucleotide analog for evaluating inhibition of viral polymerases in drug discovery programs.
• Diagnostic Assay Development: Incorporated into primers and probes to enhance specificity and enable novel detection modalities in molecular diagnostics.

Basic Information

Product Name	9-(3'-Azido-3'-Deoxy-β-D-Xylofuranosyl)Adenine 5'-Triphosphate
CAS No.	92117-27-6
Molecular Formula	C15H22N9O13P3
Molecular Weight	629.31 g/mol
Synonyms	3'-Azido-3'-deoxy-xylo-ATP; 3'-N3-xylo-ATP; 9-[(3-Azido-3-deoxy-β-D-xylofuranosyl)]adenine 5'-triphosphate; AzidoxyloATP; NTP analog, azide-modified; 3'-Azido-2',3'-dideoxy-xylo-adenosine-5'-triphosphate; Chain-terminating nucleotide triphosphate
EINECS	Contact for details

Quality Control

Every batch of 9-(3'-Azido-3'-Deoxy-β-D-Xylofuranosyl)Adenine 5'-Triphosphate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency, critical for sensitive research applications. Our quality protocols are designed to meet the exacting standards of life science research. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Triethylammonium Salt Content	≤ 10%
Biological Activity	Suitable for use as polymerase substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.