Description

Lambda Phage DNA is a high-purity, linear double-stranded DNA derived from the bacteriophage lambda, a standard model organism in molecular biology. This product is essential for research and development, serving as a critical tool for genomic mapping, restriction enzyme analysis, and as a size standard in gel electrophoresis. It is primarily utilized by biotechnology companies, academic research institutions, and diagnostic kit manufacturers engaged in genetic engineering, quality control of molecular biology reagents, and next-generation sequencing workflows.

Application

• Molecular Biology Research: Used as a standard substrate for restriction enzyme digestion, ligation assays, and DNA packaging studies.
• Genomic Analysis: Serves as a critical size marker and calibration standard in agarose and pulsed-field gel electrophoresis (PFGE).
• Next-Generation Sequencing (NGS): Employed as a control DNA for validating library preparation protocols and sequencing platform performance.
• Diagnostic Kit Manufacturing: Integrated as a positive control or reference material in PCR-based diagnostic kits and assays.
• Biotechnology Development: Acts as a template for in vitro transcription, the production of hybridization probes, and recombinant DNA technology.
• Quality Control: Provides a benchmark for assessing the activity and specificity of nucleases, polymerases, and other DNA-modifying enzymes.

Basic Information

Product Name	Lambda Phage DNA
CAS No.	91080-14-7
Molecular Formula	N/A (Biological Polymer)
Molecular Weight	~3.2 x 107 Da (48,502 base pairs)
Synonyms	Bacteriophage Lambda DNA; λ DNA; λ-Phage DNA; Phage Lambda DNA; Lambda DNA; Enterobacteria phage lambda DNA; Cl857 Sam7 DNA; DNA, Lambda Phage; DNA, Phage Lambda
EINECS	Contact for details

Quality Control

Our Lambda Phage DNA is produced and tested under stringent conditions to ensure batch-to-batch consistency and performance reliability for critical research applications. Each lot is characterized for integrity, purity, and concentration. A comprehensive Certificate of Analysis (COA) detailing specifications such as A260/A280 ratio, absence of contaminating nucleases, and restriction digest pattern is provided to guarantee it meets the high standards required for molecular biology and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquot as recommended to maintain optimal integrity and prevent shearing.

Specification

Item	Specification
Appearance	Clear, viscous solution
Concentration	0.3 - 0.7 mg/mL (in TE Buffer)
Purity (A260/A280)	1.8 - 2.0
Identification (Gel Electrophoresis)	Single band corresponding to ~48.5 kbp
Integrity (Restriction Digest)	Yields expected fragment pattern with EcoRI, HindIII, etc.
Nuclease Activity	Not detected
Protein Contamination	Not detected
RNA Contamination	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.