Description

T4 Dna Ligase is a highly specific enzyme that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA. This product is essential for molecular biology research, enabling the efficient joining of DNA fragments. It is a critical reagent for researchers and professionals in biotechnology, pharmaceutical development, and academic laboratories working on gene cloning, sequencing, and nucleic acid assembly.

Application

• Molecular Cloning: Ligation of DNA inserts into plasmid, cosmid, or viral vectors.
• Next-Generation Sequencing (NGS) Library Preparation: Joining of adapters to DNA fragments for sequencing workflows.
• Site-Directed Mutagenesis: Sealing nicks in circular DNA following primer extension.
• DNA Repair and Recombination Studies: Used in in vitro assays to study DNA repair pathways and homologous recombination.
• Diagnostic Assay Development: Component in kits for pathogen detection or genetic testing that require DNA ligation steps.
• Synthetic Biology: Assembly of large DNA constructs and genetic circuits from standardized biological parts (BioBricks).

Basic Information

Item	Detail
Product Name	T4 Dna Ligase
CAS No.	9015-85-4
Molecular Formula	N/A (Enzyme)
Molecular Weight	~55-68 kDa (Monomer)
Synonyms	T4 DNA Ligase; Bacteriophage T4 DNA Ligase; ATP-dependent DNA Ligase; Polydeoxyribonucleotide synthase (ATP); DNA Joinase; EC 6.5.1.1; T4 Polynucleotide Ligase
EINECS	Contact for details

Quality Control

Our T4 Dna Ligase is produced under stringent conditions to ensure high activity, purity, and lot-to-lot consistency. Each batch is tested for functional activity in standard ligation assays and screened for contaminating nuclease activities. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. The enzyme is supplied in a storage buffer containing glycerol. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Specific Activity	≥ 200,000 cohesive end ligation units (U) per mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Activities	Exonuclease: Not detectable Endonuclease: Not detectable
Functional Test	Efficient ligation of cohesive and blunt ends under recommended conditions
Storage Buffer	20 mM Tris-HCl, 1 mM DTT, 50 mM KCl, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.