Description

6-Tet Phosphoramidite [5'-Tetrachlorofluorescein Phosphoramidite] is a high-purity, fluorescently-labeled phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is specifically engineered to introduce a 6-Tetrachlorofluorescein (6-TET) dye at the 5'-terminus, enabling sensitive detection and analysis in downstream applications. It is a critical reagent for molecular biology research, diagnostic assay development, and the production of labeled probes for techniques such as qPCR, FISH, and microarray analysis.

Application

• Oligonucleotide Labeling: Primary reagent for the 5'-terminal incorporation of the 6-TET fluorophore during automated DNA/RNA synthesis.
• Fluorescent Probe Synthesis: Manufacture of high-sensitivity probes for real-time quantitative PCR (qPCR) and other amplification-based detection systems.
• Diagnostic Assay Development: Key component in creating labeled oligonucleotides for clinical diagnostics, including SNP genotyping and pathogen detection.
• Fluorescence In Situ Hybridization (FISH): Production of brightly fluorescent probes for cytogenetic analysis and genomic mapping.
• Microarray and Chip Technology: Synthesis of target molecules for high-throughput genomic screening and expression analysis platforms.
• Next-Generation Sequencing (NGS): Used in the preparation of custom sequencing primers and adapters requiring specific fluorescent tags.
• Academic & Industrial Research: Fundamental tool in molecular biology laboratories for a wide range of fluorescence-based detection and analytical techniques.

Basic Information

Product Name	6-Tet Phosphoramidite [5'-Tetrachlorofluorescein Phosphoramidite]
CAS No.	877049-90-6
Molecular Formula	C47H48Cl4N3O9P
Molecular Weight	963.67 g/mol
Synonyms	6-TET Phosphoramidite; 5'-Tetrachlorofluorescein Phosphoramidite; 6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein succinimidyl ester phosphoramidite; 6-HEX Phosphoramidite (common alternate dye reference); TET Phosphoramidite; 6-TET-CE Phosphoramidite; Fluorescein-derived phosphoramidite; 5'-Fluorescent Dye Labeling Reagent
EINECS	Contact for details

Quality Control

Our 6-TET Phosphoramidite is manufactured under strict quality-controlled conditions to ensure the high purity and consistency required for sensitive synthetic applications. Each batch is rigorously analyzed by HPLC, NMR, and mass spectrometry to confirm identity, purity, and labeling efficiency. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment, ensuring reliable performance in your oligonucleotide synthesis workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under anhydrous conditions in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%
Functional Test	≥ 98.0% coupling efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.