Description

N4-(Diisobutylaminomethylidene)-5'-o-(Dimethoxytrityl)-5-(1-Propynyl)-2'-o-Methylcytidine is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its value lies in enabling the precise incorporation of modified cytidine bases, which is essential for advanced research and therapeutic development. This compound is primarily needed by biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA development, and diagnostic probe manufacturing, where sequence specificity and stability are paramount.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of modified DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Development: For creating therapeutic molecules that target specific RNA sequences to modulate gene expression.
• siRNA and miRNA Research: Used in the production of small interfering RNA (siRNA) and microRNA (miRNA) analogs with enhanced nuclease resistance and binding affinity.
• Diagnostic Probe Manufacture: Essential for crafting high-specificity probes used in PCR, FISH, and microarray technologies.
• Structural Biology Studies: Incorporation into oligonucleotides for NMR and X-ray crystallography to study nucleic acid structure and protein interactions.
• Aptamer Development: For generating chemically modified aptamers with improved stability and target-binding properties for therapeutic and sensing applications.

Basic Information

Product Name	N4-(Diisobutylaminomethylidene)-5'-o-(Dimethoxytrityl)-5-(1-Propynyl)-2'-o-Methylcytidine
CAS No.	869355-38-4
Molecular Formula	C46H56N4O6
Molecular Weight	760.97 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-5-(1-propynyl)-N4-(diisobutylaminomethylene)cytidine; 2'-O-Methyl-5-(1-propynyl)cytidine Phosphoramidite (DMT-off); 5-Propynyl-2'-O-methylcytidine Phosphoramidite derivative; N4-(Diisobutylaminomethylene)-5'-O-DMT-5-propynyl-2'-O-methylcytidine; 2'-O-Me-5-propynyl-C Phosphoramidite; 5-Propynyl-dC(2'-OMe) Phosphoramidite; Advanced nucleic acid monomer; Modified cytidine building block
EINECS	Contact for details

Quality Control

Every batch of this high-value phosphoramidite is produced and tested under strict quality management systems to ensure consistency and reliability for sensitive oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and moisture content. Our quality protocols are designed to meet the exacting standards required for GMP-grade manufacturing and research applications, ensuring optimal coupling efficiency and yield in your synthesis processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.