Description

5'-O-(Dimethoxytrityl)-2-(Dimethylaminomethylidene-Amino)Purine-2'-Deoxyriboside is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of modified adenine bases into DNA sequences, which is essential for advanced research and therapeutic development. This compound is specifically designed for use by researchers and manufacturers in the fields of pharmaceutical development, molecular diagnostics, and biotechnology, where reliable, high-quality synthetic intermediates are paramount.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of DNA and RNA strands, particularly those requiring modified adenine bases.
• Antisense Therapy Development: Used in the research and production of antisense oligonucleotides (ASOs) designed to target specific RNA sequences for therapeutic intervention.
• siRNA and miRNA Synthesis: A crucial component in manufacturing small interfering RNA (siRNA) and microRNA (miRNA) for gene silencing and functional genomics studies.
• Molecular Probes and Primers: Enables the creation of labeled or modified DNA probes and primers for advanced PCR, sequencing, and hybridization applications.
• Aptamer Development: Used in the synthesis of DNA aptamers, which are single-stranded oligonucleotides engineered to bind specific target molecules.
• Research Scale cGMP Manufacturing: Serves as a starting material for the synthesis of active pharmaceutical ingredients (APIs) in oligonucleotide-based drugs under controlled conditions.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-2-(Dimethylaminomethylidene-Amino)Purine-2'-Deoxyriboside
CAS No.	869354-94-9
Molecular Formula	C34H37N7O5
Molecular Weight	623.71 g/mol
Synonyms	2-(Dimethylaminomethyleneamino)-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine; 2'-Deoxy-2-(dimethylaminomethyleneamino)-5'-O-(4,4'-dimethoxytrityl)adenosine; DMT-dA(DMF) Amidite precursor; N2-(Dimethylaminomethylene)-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)guanosine (common mis-assignment check); 2'-Deoxyadenosine derivative, DMT-protected; N2-DMF-dA(DMT) (abbreviation); 5'-O-DMT-N2-DMF-2'-deoxyadenosine
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive synthetic applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes. Our quality management system is designed to support research and development (R&D) through to pre-clinical cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions, preferably in a controlled atmosphere glovebox or using standard Schlenk techniques, to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.