Description

Restriction Endonuclease Eco 47Iii is a highly specific type II restriction enzyme that recognizes and cleaves DNA at a defined palindromic sequence. This precision tool is critical for ensuring accurate and reproducible results in molecular biology workflows, from basic research to advanced genetic engineering. It is an essential reagent for molecular biologists, geneticists, and researchers in the biotechnology and pharmaceutical industries who require reliable DNA manipulation for cloning, genotyping, and synthetic biology applications.

Application

• Molecular Cloning: Essential for precise DNA fragment excision and vector linearization in recombinant DNA technology.
• Genotyping and DNA Mapping: Used in restriction fragment length polymorphism (RFLP) analysis for genetic fingerprinting and mutation detection.
• Plasmid Construction and Validation: Critical for verifying plasmid integrity and confirming successful insertion of DNA fragments.
• Synthetic Biology: Employed in the assembly of genetic circuits and standardized biological parts (BioBricks).
• Diagnostic Assay Development: Used to create specific DNA cleavage patterns for pathogen detection and genetic disease screening.
• Genome Editing Support: Facilitates the analysis and validation of CRISPR/Cas9 and other genome editing outcomes.

Basic Information

Product Name	Restriction Endonuclease Eco 47Iii
CAS No.	88086-22-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Eco47III; Endodeoxyribonuclease Eco47III; Restriction enzyme Eco47III; Type II restriction enzyme Eco47III; EC 3.1.21.4; Enzyme Commission number 3.1.21.4; AGG^CCT recognition enzyme
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Eco 47Iii is produced under stringent quality management systems to ensure batch-to-batch consistency and optimal performance. Each lot undergoes rigorous testing for specific activity, purity (free from non-specific nucleases and phosphatases), and star activity inhibition. Certificates of Analysis (COA) detailing activity units, buffer compatibility, and storage stability are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in the supplied glycerol-containing storage buffer. Avoid repeated freeze-thaw cycles. Keep the enzyme on ice or in a chilled block during use.

Specification

Item	Specification
Appearance	Clear colorless solution
Identification (Activity)	Cleaves lambda DNA at the AGG^CCT recognition site
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95% single band
Non-Specific Nuclease Activity	Not detected
Phosphatase Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.