Description

Restriction Endonuclease Ban I From Bacillus aneurinolyticus is a highly specific Type II restriction enzyme that recognizes and cleaves the palindromic DNA sequence 5′-G↓GYRCC-3′ (where Y = C/T, R = A/G), generating cohesive ends. It is essential for precise DNA manipulation in molecular cloning, genotyping, and genome mapping workflows. This enzyme is widely used by research laboratories, biotechnology companies, and contract development and manufacturing organizations (CDMOs) requiring reproducible, high-fidelity enzymatic digestion under standardized buffer conditions. The product referenced as Restriction Endonuclease Ban I From*Baci Llus Aneuri CAS NO 85876-07-9 is supplied as a purified, lyophilized or liquid formulation optimized for industrial-scale nucleic acid processing.

Application

• Molecular cloning and plasmid construction in academic and industrial R&D labs
• Restriction fragment length polymorphism (RFLP) analysis for genetic variation studies
• Quality control of recombinant DNA constructs in biopharmaceutical process development
• Preparation of defined DNA fragments for next-generation sequencing (NGS) library construction
• Site-directed mutagenesis and gene editing vector assembly
• Diagnostic assay development for pathogen detection and SNP identification
• Production of standardized DNA ladders and molecular weight markers
• Enzyme-based quality assurance in GMP-compliant nucleic acid manufacturing facilities

Basic Information

Product Name	Restriction Endonuclease Ban I From Bacillus aneurinolyticus
CAS No.	85876-07-9
Molecular Formula	Not applicable (protein/enzyme)
Molecular Weight	~32,000 Da (monomeric subunit; exact value varies by strain and purification)
Synonyms	BanI; Ban I; BAN I; Restriction enzyme BanI; EC 3.1.21.4; Bacillus aneurinolyticus restriction endonuclease I; BanI methyltransferase inhibitor target; Type II restriction enzyme BanI; 5′-GGRYCC-3′ endonuclease; BanI DNA cleavage enzyme
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is validated for specific activity (≥10,000 units/mg protein), absence of contaminating nucleases (DNase, RNase, protease), lot-to-lot consistency, and sterility (for sterile-filtered formulations). Testing follows ISO 9001–certified protocols and aligns with recommendations from the New England Biolabs (NEB) and Thermo Fisher Scientific restriction enzyme quality benchmarks. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at –20°C for long-term stability; short-term storage (up to 4 weeks) may be performed at 2–8°C. Avoid repeated freeze-thaw cycles. Keep desiccated when in lyophilized form. Maintain relative humidity below 40% during storage.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear colorless to pale yellow liquid solution
Activity	≥10,000 units/mg protein (1 unit cleaves 1 µg λ DNA in 1 hour at 37°C)
Specific Activity	≥500 units/µL (liquid format, typical)
Protein Concentration	0.5–2.0 mg/mL (liquid format)
Identification (SDS-PAGE)	Single band at ~32 kDa
Identification (Mass Spectrometry)	Confirms primary amino acid sequence
Assay	HPLC (purity ≥95%)
Residual Solvents	Compliant with ICH Q3C guidelines
Endotoxin Level	<1.0 EU/mg (for cell culture–grade formulations)
Microbial Contamination	Absent (USP <61> / EP 2.6.12)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.