Description

Poly(D(G-T).D(C-A)) is a synthetic, double-stranded DNA oligonucleotide with a specific alternating purine-pyrimidine sequence. This compound is a critical research-grade material valued for its defined structure and high purity, making it an essential tool for molecular biology and biochemical studies. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in nucleic acid research, hybridization assays, and the development of molecular probes.

Application

• Molecular Biology Research: Used as a model duplex DNA substrate for studying DNA-protein interactions, enzyme kinetics (e.g., nucleases, polymerases), and structural analysis.
• Diagnostic Assay Development: Serves as a positive control, calibrator, or capture probe in the development of PCR, qPCR, and other nucleic acid amplification tests (NAATs).
• Biophysical Studies: Employed in spectroscopic analyses (e.g., UV melting curves, CD spectroscopy) to investigate DNA stability, hybridization thermodynamics, and conformational properties.
• Sensor and Nanotechnology: Acts as a building block or recognition element in the fabrication of biosensors and DNA-based nanomaterials due to its predictable base-pairing.
• Pharmaceutical Screening: Utilized in high-throughput screening assays to identify compounds that bind to or alter the structure of double-stranded DNA.

Basic Information

Product Name	Poly(D(G-T).D(C-A))
CAS No.	85824-40-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Poly[d(G-T)•d(C-A)]; Poly(deoxyguanylyl-thymidylic acid) • Poly(deoxycytidylyl-adenylic acid); Poly(dG-dT)•Poly(dC-dA); Alternating GT/CA DNA Polymer; Synthetic DNA Duplex (GT/CA); Poly[d(GT)•d(CA)]; DNA Oligonucleotide Duplex 85824-40-4
EINECS	Contact for details

Quality Control

Our Poly(D(G-T).D(C-A)) is manufactured under controlled conditions to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment, UV spectroscopy for concentration and duplex confirmation, and gel electrophoresis for integrity. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation and moisture uptake. Aliquot into smaller volumes after initial thawing to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (UV)	Characteristic absorption spectrum for double-stranded DNA
Purity (HPLC)	≥ 95% (Duplex form)
Absorbance Ratio (A260/A280)	1.8 - 2.0
Molecular Weight Confirmation	By Mass Spectrometry (MALDI-TOF or ESI)
Endotoxin Level	< 10 EU/mg (for research-grade)
Solubility	Soluble in TE buffer or nuclease-free water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.