Description

3'-o-(n-Methylanthraniloyl)Adenosine 5'-Diphosphate CAS NO 85287-55-4 is a specialized fluorescently labeled nucleotide analog, where an N-methylanthraniloyl (MANT) fluorophore is attached to the ribose ring of adenosine diphosphate. This modification is critical for creating a sensitive, non-radioactive probe for studying enzyme kinetics and nucleotide-protein interactions. It is primarily utilized by researchers and developers in biochemical research, enzymology, and high-throughput screening for drug discovery and diagnostic assay development.

Application

• Enzyme Kinetics & Mechanism Studies: As a fluorescent substrate for monitoring the activity of ATPases, kinases, and other nucleotide-binding enzymes in real-time.
• High-Throughput Screening (HTS): Enables the development of sensitive, homogeneous assays for identifying enzyme inhibitors or activators in drug discovery campaigns.
• Protein-Ligand Binding Assays: Used in fluorescence polarization (FP) or fluorescence resonance energy transfer (FRET) assays to study binding affinities and conformational changes.
• Diagnostic Assay Development: Serves as a key component in developing research-grade and prototype diagnostic tests for various diseases.
• Biochemical Research Reagent: A vital tool for academic and industrial research into nucleotide signaling pathways and metabolic processes.

Basic Information

Product Name	3'-o-(n-Methylanthraniloyl)Adenosine 5'-Diphosphate
CAS No.	85287-55-4
Molecular Formula	C23H25N6O13P2
Molecular Weight	655.42 g/mol
Synonyms	MANT-ADP; 3'-O-(N-Methylanthraniloyl)adenosine-5'-diphosphate; 3'-O-(N-Methyl-2-aminobenzoyl)adenosine 5'-diphosphate; Adenosine 5'-(trihydrogen diphosphate), 3'-ester with N-methylanthranilic acid; 3'-O-(N-Methylanthraniloyl)-ADP; Fluorescent ADP analog; MANT-labeled ADP
EINECS	Contact for details

Quality Control

Every batch of 3'-o-(n-Methylanthraniloyl)Adenosine 5'-Diphosphate is manufactured and tested under stringent quality protocols to ensure high purity, consistent fluorescence properties, and reliable performance in sensitive assays. Our quality system includes comprehensive analytical characterization using HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) providing purity, identity, and fluorescence data is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95%
Assay (UV-Vis)	≥ 90% (anhydrous basis)
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Excitation ~356 nm, Emission ~448 nm (in aqueous buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.