Description

Biotin-5-Cytidine-5'-Triphosphate Lithium Salt - 1 Mm Aqueous Solution is a chemically modified nucleotide, specifically a biotinylated cytidine triphosphate analog, supplied as a ready-to-use 1 mM aqueous solution. This product is essential for enzymatic labeling and detection applications, offering a convenient and standardized format that eliminates the need for complex reconstitution and reduces handling errors. It is primarily used by researchers and manufacturers in the life sciences, molecular diagnostics, and next-generation sequencing (NGS) sectors for sensitive probe generation and nucleic acid detection.

Application

• Next-Generation Sequencing (NGS): Enzymatic incorporation for the preparation of biotin-labeled DNA libraries and probes.
• Microarray Technology: Synthesis of labeled cDNA or cRNA targets for hybridization on gene expression or SNP arrays.
• In Situ Hybridization (ISH/FISH): Generation of high-sensitivity, non-radioactive probes for chromosomal and tissue analysis.
• DNA/RNA Labeling (Nick Translation, Random Priming): A critical substrate for creating biotin-tagged nucleic acids for blotting and pull-down assays.
• Protein-Nucleic Acid Interaction Studies: Used in assays like EMSA (Electrophoretic Mobility Shift Assay) for detecting protein binding with streptavidin-based detection.
• Diagnostic Assay Development: A key component in the manufacture of kits for pathogen detection and genetic testing.

Basic Information

Product Name	Biotin-5-Cytidine-5'-Triphosphate Lithium Salt - 1 Mm Aqueous Solution
CAS No.	85231-47-6
Molecular Formula	C₂₀H₂₈Li₄N₇O₁₇P₃S
Molecular Weight	823.24 g/mol (Free acid form, approximate)
Synonyms	Biotin-16-dCTP; Biotin-16-2'-deoxycytidine-5'-triphosphate; 5-(N-(Biotinoyl)-aminohexyl)-2'-deoxycytidine-5'-triphosphate; Biotin-dCTP; Biotinylated dCTP; Biotin-16-AA-dCTP; CTP, 5'-(trihydrogen diphosphate), 5'-5'-ester with 5-(6-((5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl)amino)hexyl)cytidine
EINECS	Contact for details

Quality Control

Our Biotin-5-Cytidine-5'-Triphosphate Lithium Salt solution is manufactured under strict quality systems. Each batch is tested to ensure high purity, correct concentration, and functional performance. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications for concentration (UV spectroscopy), purity (HPLC), identity, pH, and absence of contaminating nucleases. We support compliance with relevant ISO 13485 and GMP guidelines for diagnostic and research use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C. Avoid repeated freeze-thaw cycles. Aliquot as needed to maintain product integrity.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow aqueous solution
Concentration	1.0 mM ± 10%
Purity (HPLC)	≥ 95%
Identification (UV)	Spectrum consistent with reference
pH	6.5 - 7.5
Endotoxin	< 0.1 EU/µmol (if specified)
Functional Test	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.