Description

Restriction Endonuclease Gsu I is a highly specific type II restriction enzyme that recognizes and cleaves DNA at a defined nucleotide sequence. This precision is critical for molecular biology applications requiring accurate DNA manipulation and analysis. It is an essential tool for researchers and technicians in biotechnology, pharmaceutical development, and academic research laboratories working on genetic engineering, cloning, and genomic mapping.

Application

• Molecular Cloning: Essential for cutting DNA at specific sites to insert genes into plasmid vectors.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used in genetic fingerprinting and mutation detection.
• Genomic DNA Mapping: Aids in constructing physical maps of genomes by generating defined DNA fragments.
• Recombinant DNA Technology: Fundamental for constructing recombinant DNA molecules in bioprocess development.
• Diagnostic Assay Development: Employed in creating tests for genetic disorders and pathogen detection.
• Site-Directed Mutagenesis: Facilitates the precise alteration of DNA sequences for functional studies.

Basic Information

Product Name	Restriction Endonuclease Gsu I
CAS No.	85130-39-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	GsuI; Endodeoxyribonuclease GsuI; Gsu I Restriction Enzyme; Restriction Enzyme Gsu I; GsuI Endonuclease; EC 3.1.21.4; Type II Restriction Enzyme GsuI
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Gsu I is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and absence of contaminating nucleases. Certificates of Analysis (COA) detailing activity units, purity assays, and functional testing in standard buffers are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. The enzyme is supplied in a storage buffer containing glycerol. Avoid repeated freeze-thaw cycles to maintain optimal activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Activities	Devoid of detectable exo- and endonuclease, phosphatase
Functional Test	Complete digestion of lambda DNA in 1 hour
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.