Description

Restriction Endodeoxyribonuclease Paer7 is a highly specific type II restriction enzyme isolated from *Pseudomonas aeruginosa*. This enzyme is critical for molecular biology research, enabling precise DNA cleavage at its unique recognition sequence. It is an essential tool for genetic engineering, recombinant DNA technology, and genomic analysis workflows. Researchers in biotechnology, pharmaceutical development, and academic institutions rely on its specificity and high activity for cloning, mapping, and sequencing applications.

Application

• Recombinant DNA Technology: Essential for cutting and inserting DNA fragments in plasmid construction and gene cloning.
• Genomic DNA Analysis: Used in restriction mapping and Southern blotting to analyze genetic structure and mutations.
• Molecular Diagnostics: Employed in RFLP (Restriction Fragment Length Polymorphism) analysis for pathogen detection and genetic fingerprinting.
• Synthetic Biology: Facilitates the assembly of genetic circuits and synthetic pathways by providing specific DNA cleavage sites.
• Biopharmaceutical Production: Used in the development and quality control of genetically engineered cell lines for therapeutic protein production.
• Academic & Industrial Research: A fundamental reagent for basic research in genetics, microbiology, and cellular biology.

Basic Information

Product Name	Restriction Endodeoxyribonuclease Paer7
CAS No.	84522-61-2
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	Contact for details
Synonyms	Paer7 Restriction Enzyme; Endodeoxyribonuclease Paer7; Type II Restriction Enzyme Paer7; Restriction Enzyme from *Pseudomonas aeruginosa*; DNA Restriction Enzyme Paer7; REase Paer7; Paer7 REase; *Pae*R7I (common nomenclature)
EINECS	Contact for details

Quality Control

Our Restriction Endodeoxyribonuclease Paer7 is produced and tested under stringent conditions to ensure exceptional purity, specificity, and activity. Each lot undergoes rigorous analytical testing, including assays for endonuclease activity, absence of non-specific nuclease and phosphatase activities, and stability profiling. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity (units/mg), and storage buffer composition is provided with every shipment to guarantee batch-to-batch consistency for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in the supplied storage buffer. The product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Aliquotting upon receipt is recommended for frequent use to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves specific DNA recognition sequence
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Non-Specific Nuclease Activity	Not detected
Phosphatase Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λ DNA in 1 hour at 37°C in the recommended reaction buffer.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.