Description

Restriction Endonuclease Ban II from *Bacillus aneurinolyticus* is a highly specific type II restriction enzyme that recognizes and cleaves the palindromic DNA sequence 5'-GRGCYC-3'. This precision tool is essential for molecular biology research, enabling the manipulation and analysis of genetic material with high fidelity. It is a critical reagent for biotechnology companies, academic research laboratories, and diagnostic developers working in genetic engineering, cloning, and DNA mapping.

Application

• Recombinant DNA Technology: Essential for precise DNA cleavage in cloning and vector construction.
• Genetic Mapping: Used to generate restriction fragment length polymorphisms (RFLPs) for genome analysis.
• Molecular Diagnostics: Employed in the development of assays for genetic disorder screening and pathogen detection.
• Biopharmaceutical Production: Aids in the genetic modification of cell lines for therapeutic protein expression.
• Academic & Industrial Research: Fundamental tool for studying gene structure, function, and regulation.
• Synthetic Biology: Used in the assembly of genetic circuits and synthetic genomes.

Basic Information

Product Name	Restriction Endonuclease Ban II from *Bacillus aneurinolyticus*
CAS No.	84067-33-4
Molecular Formula	Protein
Molecular Weight	Contact for details
Synonyms	Ban II Restriction Enzyme; Endodeoxyribonuclease BanII; Type II Restriction Enzyme BanII; EC 3.1.21.4; BanII; Restriction Endonuclease BanII (from *Bacillus aneurinolyticus*); Deoxyribonuclease BanII
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Ban II is produced and tested under stringent conditions to ensure high activity, specificity, and absence of contaminating nucleases. Each lot undergoes rigorous analytical testing, including activity assays, purity assessment, and tests for non-specific nicking. We provide comprehensive Certificates of Analysis (COA) detailing lot-specific performance data, including units of activity and storage buffer composition, to support your research and quality documentation requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in the supplied glycerol-containing storage buffer. Avoid repeated freeze-thaw cycles. Keep the enzyme on ice or in a chilled block during use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves 1 µg λ DNA in 1 hour at 37°C
Specific Activity	≥ 5,000 units/mg protein
Purity (SDS-PAGE)	≥ 90% single band
Exonuclease Activity	Not detectable
Endonuclease Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.