Description

8-Azidoadenosine-5'-Triphosphate 2',3'-Dialdehyde is a chemically modified nucleotide analog that serves as a critical cross-linking and photoaffinity labeling reagent. This compound matters for researchers because it provides a unique bifunctional handle, allowing for the covalent attachment of ATP-binding proteins or enzymes for structural and functional studies. It is primarily needed by scientists in biochemistry, molecular biology, and medicinal chemistry for investigating nucleotide-binding sites, enzyme mechanisms, and developing targeted probes.

Application

• Photoaffinity Labeling: Used to irreversibly tag and identify ATP-binding proteins and active sites in complex biological mixtures upon UV irradiation.
• Bioconjugation and Cross-linking: Serves as a key reagent for creating stable covalent bonds between biomolecules, facilitating the study of protein-protein or protein-nucleic acid interactions.
• Enzyme Mechanism Studies: Employed to probe the structure and function of kinases, ATPases, and other ATP-dependent enzymes by acting as a mechanism-based inhibitor.
• Probe Development: Utilized in the synthesis of advanced molecular probes for fluorescence, affinity purification, or diagnostic applications.
• Structural Biology: Aids in capturing transient complexes for analysis by techniques like X-ray crystallography or cryo-electron microscopy.
• Drug Discovery Research: Applied in high-throughput screening and target validation for diseases involving purinergic signaling.

Basic Information

Product Name	8-Azidoadenosine-5'-Triphosphate 2',3'-Dialdehyde
CAS No.	83700-69-0
Molecular Formula	C₁₀H₁₂N₈O₁₂P₃
Molecular Weight	577.16 g/mol
Synonyms	8-N₃-ATP-2',3'-dialdehyde; 8-Azido-ATP dialdehyde; Periodate-oxidized 8-Azido-ATP; 8-Azidoadenosine triphosphate dialdehyde; 2',3'-Dialdehyde-8-azido-ATP; Reactive ATP analog; Photoactivatable ATP analog; ATP cross-linking reagent
EINECS	Contact for details

Quality Control

Our 8-Azidoadenosine-5'-Triphosphate 2',3'-Dialdehyde is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic verification of structure and functional groups. A detailed Certificate of Analysis (COA) documenting identity, purity, and stability is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or elevated temperatures will lead to decomposition. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Tested in standard cross-linking assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.