Description

Restriction Endonuclease Sau 96 I is a highly specific type II restriction enzyme that recognizes and cleaves the DNA sequence 5'-G^GNCC-3'. This product is essential for molecular biology research, enabling precise DNA manipulation, mapping, and cloning. It is a critical reagent for biotechnology companies, academic research laboratories, and diagnostic kit manufacturers involved in genetic engineering and analysis.

Application

• Molecular Cloning: Essential for the precise cutting and insertion of DNA fragments into vectors.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used for genetic fingerprinting, mutation detection, and genotyping.
• DNA Mapping: Key tool for constructing physical maps of genomes and plasmids.
• Recombinant DNA Technology: Facilitates the creation of recombinant DNA molecules for protein expression and functional studies.
• Diagnostic Assay Development: Employed in the design and production of kits for detecting specific genetic sequences or mutations.
• Genome Editing Support: Used in preparatory steps for techniques like CRISPR, such as vector linearization.

Basic Information

Product Name	Restriction Endonuclease Sau 96 I
CAS No.	81811-57-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Sau96I; Endodeoxyribonuclease Sau96I; Sau 96I; Restriction enzyme Sau96I; Type II restriction enzyme Sau96I; Sau96I endonuclease; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Sau 96 I is produced and tested under stringent quality management systems to ensure high purity, specific activity, and absence of contaminating nucleases. Each batch is rigorously tested for activity units, star activity, and specificity. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and storage buffer composition is provided with every shipment to guarantee batch-to-batch consistency and performance reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer containing 50% glycerol.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Activities	Absence of detectable exo- and endonuclease, phosphatase, and protease activities
Recognition Sequence	5'-G^GNCC-3'
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.