Description

Guanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is a chemically modified nucleotide analog where a non-bridging oxygen atom in the α-phosphate group is replaced by sulfur, creating a chiral center and resulting in the Rp diastereomer. This compound is a critical biochemical tool, serving as a hydrolysis-resistant GTP analog that can be used to probe and manipulate G-protein and GTPase signaling pathways. It is essential for researchers and manufacturers in molecular biology, enzymology, and pharmaceutical development, particularly for studying protein function, signal transduction, and high-throughput screening assays.

Application

• G-protein and GTPase Research: Used as a non-hydrolyzable GTP analog to study the activation and regulation of G-proteins, small GTPases (e.g., Ras, Rho, Rab families), and other GTP-binding proteins.
• Enzyme Kinetics and Mechanism Studies: Employed in assays to investigate the kinetics, specificity, and catalytic mechanisms of nucleotide-dependent enzymes, including kinases, polymerases, and ATPases.
• Structural Biology: Facilitates the crystallization and structural analysis of GTP-bound protein complexes by providing a stable, hydrolysis-resistant ligand.
• High-Throughput Screening (HTS): Acts as a key reagent in drug discovery campaigns targeting oncogenic Ras mutants and other disease-relevant GTPases.
• Signal Transduction Pathway Analysis: Essential for elucidating cellular signaling pathways that rely on GTP-binding proteins as molecular switches.
• Diagnostic Kit Development: Incorporated into specialized biochemical kits for the detection and quantification of GTPase activity.

Basic Information

Product Name	Guanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt
CAS No.	81570-51-6
Molecular Formula	C10H14N5O13P3S · xNa+
Molecular Weight	Approx. 567.1 (Free acid) + Na+
Synonyms	GTPγS, Rp isomer; Guanosine 5'-[γ-thio]triphosphate, Rp isomer sodium salt; Rp-GTPγS sodium salt; Rp-Guanosine-5'-(3-thiotriphosphate) sodium salt; (Rp)-Guanosine-5'-(γ-thiotriphosphate); Rp isomer of Guanylyl imidodiphosphate; Non-hydrolyzable GTP analog, Rp form; 5'-Guanosyl triphosphorothioate, Rp-isomer
EINECS	Contact for details

Quality Control

Our Guanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is produced under stringent conditions to ensure the highest standards of diastereomeric purity and biochemical activity. Each batch undergoes comprehensive analytical testing, including HPLC for purity and chiral analysis to confirm the Rp-isomer identity. We provide full traceability and Certificates of Analysis (COA) detailing assay, purity, and impurity profiles upon request, supporting research reproducibility and regulatory compliance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term stability, it is recommended to store under an inert atmosphere and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Diastereomeric Purity (Rp-Isomer)	≥ 98%
Assay (Enzymatic)	≥ 90%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.