Description

Thioinosine 5'-(Beta, γ-Methylene)Triphosphate (CAS NO 81536-59-6) is a chemically modified nucleotide analog, specifically a non-hydrolyzable ATP derivative. This compound is critically important for biochemical research as it acts as a potent and selective inhibitor of ATP-dependent enzymes, particularly kinases and ATPases. It is an essential tool for researchers in molecular biology, enzymology, and structural biology who require precise control over nucleotide-dependent signaling pathways.

Application

• Enzyme Inhibition Studies: Used as a non-hydrolyzable competitive inhibitor to study the mechanism and kinetics of ATP-binding enzymes, including kinases, ATPases, and GTPases.
• Structural Biology: Employed in X-ray crystallography and cryo-EM to lock target enzymes in a specific conformational state for high-resolution structural analysis.
• Signal Transduction Research: Essential for probing and dissecting complex cellular signaling cascades that rely on ATP hydrolysis, helping to elucidate disease mechanisms.
• Drug Discovery & Screening: Serves as a key biochemical tool in high-throughput screening assays to identify novel inhibitors of ATP-binding pockets in therapeutic targets.
• Biochemical Assay Development: Used as a stable reference standard and control in developing robust in vitro diagnostic and research assays.

Basic Information

Product Name	Thioinosine 5'-(Beta, γ-Methylene)Triphosphate
CAS No.	81536-59-6
Molecular Formula	C11H15N4O12P3S
Molecular Weight	520.2 g/mol
Synonyms	β,γ-Methylene-thioinosine 5'-triphosphate; 6-Thioinosine 5'-(β,γ-methylenetriphosphate); 6-Mercaptopurine riboside 5'-(β,γ-methylenetriphosphate); Adenosine 5'-(β,γ-methylenetriphosphate) thioinosine analog; Non-hydrolyzable ATP-γ-S analog; ATPγS-CH2; Inhibitor of P2X receptors; Nucleotide enzyme inhibitor
EINECS	Contact for details

Quality Control

Our Thioinosine 5'-(Beta, γ-Methylene)Triphosphate is manufactured under strict quality control protocols to ensure the high purity and consistency required for sensitive research applications. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.