Description

5-(n-(n-Biotinyl-ε-Aminocaproyl)-3-Aminoallyl)-2'-Deoxuridine 5'-Triphosphate is a specialized, biotin-labeled nucleotide analog designed for sensitive molecular detection and analysis. This compound matters because it provides a high-affinity link to streptavidin or avidin, enabling the precise tagging, isolation, and visualization of nucleic acids in complex biological systems. It is essential for researchers and developers in molecular diagnostics, next-generation sequencing (NGS) probe development, and advanced biomedical research who require reliable, high-purity reagents for critical assays.

Application

• Next-Generation Sequencing (NGS): Used as a biotinylated dUTP for the preparation of labeled probes in target enrichment, library preparation, and in-situ sequencing applications.
• Microarray Technology: Critical for synthesizing biotin-tagged cDNA or cRNA probes for hybridization onto array chips, followed by streptavidin-based signal detection.
• Fluorescence In Situ Hybridization (FISH): Enables the creation of high-sensitivity probes for chromosomal and gene mapping, where biotin-streptavidin amplification systems are employed.
• PCR and qPCR Probe Labeling: Incorporation into nucleic acid probes to facilitate capture or detection in quantitative PCR and other amplification-based diagnostic assays.
• Pull-Down Assays & Affinity Purification: Serves as a key reagent for biotinylating DNA or RNA fragments to isolate specific targets or binding partners using streptavidin-coated beads.
• Diagnostic Kit Manufacturing: A vital raw material in the production of commercial diagnostic kits for infectious diseases, genetic disorders, and oncology testing.

Basic Information

Product Name	5-(n-(n-Biotinyl-ε-Aminocaproyl)-3-Aminoallyl)-2'-Deoxuridine 5'-Triphosphate
CAS No.	81522-11-4
Molecular Formula	C₃₃H₅₀N₇O₁₉P₃
Molecular Weight	993.73 g/mol
Synonyms	Biotin-11-dUTP; Biotin-11-2'-deoxyuridine-5'-triphosphate; AA-dUTP-Biotin; 5-(N-(6-(Biotinamido)hexanoyl)-3-aminoallyl)-2'-deoxyuridine 5'-triphosphate; Aminoallyl-dUTP-Biotin; Biotinylated dUTP; 5-(N-(N-Biotinyl-ε-aminocaproyl)-3-aminoallyl)-dUTP; Biotin-11-AA-dUTP
EINECS	Contact for details

Quality Control

Our 5-(n-(n-Biotinyl-ε-Aminocaproyl)-3-Aminoallyl)-2'-Deoxuridine 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for concentration verification, and functional activity testing. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with research and diagnostic-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Spectrophotometry)	≥ 90% (as dUTP triphosphate)
Water Content (KF)	≤ 5.0%
Functional Activity	Suitable for enzymatic incorporation and streptavidin binding

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.