Description

D(Gcaatttaactgtgat) CAS NO 81412-70-6 is a defined-sequence oligonucleotide, a critical building block for advanced molecular biology and genetic research. This high-purity synthetic DNA fragment is essential for applications requiring precise genetic manipulation and sequence-specific hybridization. It is primarily utilized by biotechnology firms, academic research institutions, and diagnostic developers working on gene synthesis, probe development, and therapeutic oligonucleotide discovery.

Application

• Gene Synthesis & Cloning: Serving as a precise building block for the assembly of longer synthetic genes and plasmid construction.
• PCR & qPCR Applications: Functioning as a primer, probe, or template in polymerase chain reaction (PCR) and quantitative PCR (qPCR) assays for target amplification and detection.
• Diagnostic Probe Development: Used in the design and manufacture of specific hybridization probes for diagnostic kits targeting genetic markers or pathogens.
• Antisense Oligonucleotide (ASO) Research: A candidate sequence for investigating antisense mechanisms and developing potential therapeutic oligonucleotides.
• Site-Directed Mutagenesis: Enabling precise alterations in DNA sequences for functional genomics and protein engineering studies.
• Microarray Fabrication: Spotted onto arrays as a capture probe for high-throughput genomic analysis and expression profiling.
• Next-Generation Sequencing (NGS): Employed as a barcode, adapter, or custom sequence in NGS library preparation workflows.
• Basic Research & Teaching: A tool for fundamental molecular biology experiments and educational demonstrations in genetics.

Basic Information

Product Name	D(Gcaatttaactgtgat)
CAS No.	81412-70-6
Molecular Formula	C151H191N59O91P15
Molecular Weight	~ 4630.9 g/mol
Synonyms	Oligonucleotide d(GCAATTTAACTGTGAT); Deoxyribonucleic acid, d(GCAATTTAACTGTGAT); DNA Oligomer Sequence 5'-GCAATTTAACTGTGAT-3'; Single-stranded DNA (ssDNA) fragment; 16-mer DNA Oligo; Custom DNA Sequence; Synthetic DNA Primer/Probe; 5'-d(GCAATTTAACTGTGAT)-3'
EINECS	Contact for details

Quality Control

Our oligonucleotides are manufactured under strict quality management systems. Each batch of D(Gcaatttaactgtgat) is rigorously analyzed to ensure high purity and sequence fidelity. Standard quality assurance includes Mass Spectrometry (MS) for mass confirmation and HPLC for purity assessment. Certificates of Analysis (COA) detailing identity, purity, concentration, and quality control results are provided with every shipment and are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For short-term use, store at 2-8°C. Use ultrapure, nuclease-free water or TE buffer for reconstitution.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or pellet
Identification (Mass Spec)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95% (Can be supplied as ≥ 98% or ≥ 99%)
Sequence Verification	5'-GCAATTTAACTGTGAT-3' (Confirmed by MS)
Length	16 bases
Extinction Coefficient	~ 154,900 L/(mol·cm) at 260 nm (Calculated)
Molecular Weight	~ 4630.9 g/mol (Theoretical, average)
OD260/OD280 Ratio	1.8 - 2.0 (in water)
Endotoxin Level	< 10 EU/mg (for research-grade)
Sterility (if required)	Sterile-filtered, 0.2 µm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.