Description

Restriction Endonuclease Hha I From*Haemophilus Haemolyticus is a highly specific type II restriction enzyme that recognizes and cleaves the DNA sequence 5'-GCG↓C-3'. This precision cutting tool is essential for molecular biology research, enabling DNA manipulation, mapping, and cloning with high fidelity. It is a critical reagent for biotechnology companies, academic research laboratories, and diagnostic kit manufacturers engaged in genetic engineering and genomic analysis.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into vectors for gene expression studies and protein production.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used in genetic fingerprinting, mutation detection, and pathogen identification.
• DNA Mapping: A key tool for constructing physical maps of genomes by generating specific DNA fragments.
• Recombinant DNA Technology: Facilitates the cutting and assembly of DNA sequences in synthetic biology and genetic circuit construction.
• Diagnostic Assay Development: Employed in the design and manufacturing of kits for genetic testing and disease diagnosis.
• Epigenetics Research: Useful in studies involving DNA methylation patterns, as its activity can be sensitive to cytosine methylation within its recognition site.

Basic Information

Product Name	Restriction Endonuclease Hha I From*Haemophilus Haemolyticus
CAS No.	81295-20-7
Molecular Formula	Protein
Molecular Weight	Contact for details
Synonyms	HhaI Restriction Enzyme; Endodeoxyribonuclease HhaI; HhaI Endonuclease; Restriction Enzyme HhaI; Type II Restriction Enzyme HhaI; Haemophilus haemolyticus HhaI; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Hha I is produced under stringent quality management systems. Each batch is rigorously tested for specific activity, purity, and absence of contaminating nucleases (exonuclease and non-specific endonuclease) to ensure optimal performance in sensitive applications. Certificates of Analysis (COA) detailing lot-specific activity units, storage buffer, and quality control parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a storage buffer containing glycerol; ensure it does not freeze completely at -20°C.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cuts λ DNA at the specific 5'-GCGC-3' sequence
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Exonuclease Activity	Not detectable
Non-specific Endonuclease Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.