Description

Restriction Endonuclease Msc I From *Micr Ococcus Sp. is a high-purity, site-specific endonuclease enzyme used for precise DNA cleavage in molecular biology applications. This enzyme is critical for ensuring accurate and reproducible results in genetic engineering, cloning, and genomic analysis workflows. It is an essential reagent for biotechnology companies, pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit manufacturers requiring reliable and consistent restriction digestion.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into plasmid vectors.
• Genetic Engineering: Used in the construction of recombinant DNA molecules for protein expression and gene function studies.
• Genomic DNA Analysis: Key tool for restriction fragment length polymorphism (RFLP) mapping and Southern blotting.
• Diagnostic Assay Development: Employed in the preparation of DNA samples and probes for PCR-based diagnostics and next-generation sequencing (NGS) library prep.
• Biopharmaceutical Research: Facilitates the manipulation of genetic constructs in the development of gene therapies and vaccines.
• Academic & Industrial R&D: A fundamental reagent for basic life science research and applied biotechnology projects.

Basic Information

Product Name	Restriction Endonuclease Msc I From *Micr Ococcus Sp.
CAS No.	81295-08-1
Molecular Formula	Protein (Enzyme)
Molecular Weight	Contact for details
Synonyms	MscI; Msc I Restriction Enzyme; Endodeoxyribonuclease MscI; Type II Site-Specific Deoxyribonuclease MscI; Restriction Enzyme MscI; EC 3.1.21.4; *Micrococcus* sp. Restriction Endonuclease MscI
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Msc I is produced and tested under stringent quality management systems to ensure high activity, specificity, and absence of contaminating nucleases. Each lot undergoes rigorous analytical testing, including activity assays, purity assessment (SDS-PAGE), and tests for exonuclease and non-specific endonuclease activities. Certificates of Analysis (COA) detailing lot-specific performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; handle on ice when thawed for use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves TGGCCA sequence specifically
Activity (Units/µl)	≥ 10 U/µl (under standard assay conditions)
Purity (SDS-PAGE)	≥ 95% single band
Exonuclease Activity	Not detectable
Non-specific Endonuclease Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.