Description

5'-O-DMT-2'-O-TBDMS-N-Bz-Adenosine is a key protected nucleoside phosphoramidite intermediate, essential for the solid-phase synthesis of oligonucleotides. Its value lies in providing precise protection for the 5'-hydroxyl (DMT) and 2'-hydroxyl (TBDMS) groups, while the N6-benzoyl (N-Bz) moiety protects the adenine base, ensuring high coupling efficiency and sequence fidelity. This compound is critically needed by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for the production of antisense oligonucleotides, siRNAs, aptamers, and primers.

Application

• Solid-Phase Oligonucleotide Synthesis: As a critical building block (phosphoramidite precursor) for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and aptamer-based therapies.
• Research-Grade Oligo Synthesis: For academic and industrial R&D labs creating custom primers, probes, and model oligonucleotides.
• Diagnostic Probe Development: Synthesis of labeled or modified probes used in PCR, FISH, and microarray technologies.
• Scale-Up GMP Production: Suitable for scaling up under current Good Manufacturing Practice (cGMP) guidelines for clinical-grade material.

Basic Information

Product Name	5'-O-DMT-2'-O-TBDMS-N-Bz-Adenosine
CAS No.	81265-93-2
Molecular Formula	C48H55N5O7Si
Molecular Weight	842.08 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N6-benzoyladenosine; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-[(1,1-dimethylethyl)dimethylsilyl]adenosine; DMT-TBDMS-N-Bz-Adenosine; Adenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]]-, 6-benzoate; Protected Adenosine Derivative; 2'-O-TBDMS-5'-O-DMT-N6-Bz-Adenosine
EINECS	Contact for details

Quality Control

Our 5'-O-DMT-2'-O-TBDMS-N-Bz-Adenosine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive oligonucleotide synthesis. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to industry-standard analytical methods, and material can be supplied to meet specific research or cGMP-grade requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, dichloromethane, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.