Description

Poly(G,A).Poly(C) CAS NO 81251-17-4 is a synthetic, double-stranded polynucleotide complex composed of guanine, adenine, and cytosine bases. This compound is a critical reagent in molecular biology and biochemistry research, serving as a defined nucleic acid structure for studying protein-nucleic acid interactions and enzymatic mechanisms. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in advanced life science applications, including the development of antiviral and gene therapy strategies.

Application

• Biochemical Research: Used as a defined substrate or template for studying DNA/RNA polymerases, nucleases, helicases, and other nucleic acid-modifying enzymes.
• Diagnostic Assay Development: Serves as a control or calibrator in the development of PCR-based assays, hybridization probes, and other molecular diagnostic tests.
• Antiviral Research: Investigated for its potential to interact with viral polymerases or as a model for studying nucleic acid-based antiviral compounds.
• Structural Biology: Provides a standardized double-stranded nucleic acid for X-ray crystallography, NMR spectroscopy, and other structural analysis techniques.
• Gene Regulation Studies: Employed in vitro studies to analyze transcription factor binding, gene silencing mechanisms, and chromatin dynamics.
• Quality Control Reagent: Acts as a process control in the manufacturing of enzymes and kits used for nucleic acid amplification and analysis.

Basic Information

Product Name	Poly(G,A).Poly(C)
CAS No.	81251-17-4
Molecular Formula	(C10H12N5O3P • C5H5N5O • C9H12N3O6P)n
Molecular Weight	Variable (Polymer)
Synonyms	Poly(guanylic, adenylic) • poly(cytidylic) acid complex; Poly(G,A)-Poly(C); Poly r(GA) • Poly r(C); Polyriboguanylic-adenylic acid • Polyribocytidylic acid; Poly(GA) • Poly(C); Double-stranded RNA analog [poly(GA:UC)]; Synthetic dsRNA polymer; Polyribonucleotide complex
EINECS	Contact for details

Quality Control

Our Poly(G,A).Poly(C) is produced under stringent conditions to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is characterized for base composition, strand integrity, and absence of nucleases. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications such as UV absorbance ratios (A260/A280), melting temperature (Tm), and endotoxin levels. We adhere to ISO 9001 quality management principles in our manufacturing processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated. Aliquot into single-use vials after reconstitution to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solid
Identification (UV-Vis)	Characteristic absorption spectrum for polynucleotides
Identification (IR)	Spectrum consistent with reference
Assay (A260)	≥ 90% (by UV absorbance)
Base Ratio (G:A:C)	Approximately 1:1:1 (by HPLC)
Purity (HPLC)	≥ 95% (Polymer peak)
Solubility	Soluble in TE buffer or nuclease-free water
Endotoxin	< 10 EU/mg (where specified)
Nuclease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.