Description

5'-O-(4,4'-Dimethoxytrityl)-3'-O-T-Butyldimethylsilyl Uridine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides. This compound provides critical protection for both the 5'- and 3'-hydroxyl groups of uridine, enabling precise and high-yield coupling reactions in automated synthesizers. It is a fundamental building block for researchers and manufacturers in the pharmaceutical and biotechnology sectors developing oligonucleotide-based therapeutics, including antisense drugs, siRNA, and aptamers.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A primary phosphoramidite precursor for the automated synthesis of RNA and modified DNA strands.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs targeting specific mRNA sequences.
• Small Interfering RNA (siRNA) Manufacturing: A critical raw material for constructing siRNA duplexes used in gene silencing applications.
• Aptamer and Diagnostic Probe Synthesis: Enables the creation of high-affinity nucleic acid ligands and labeled probes for diagnostics.
• Research-Scale Nucleoside Chemistry: Serves as a versatile intermediate for academic and industrial research into novel nucleoside analogs and prodrugs.
• GMP-Grade Therapeutic Production: Supplied under controlled conditions for use in Good Manufacturing Practice (GMP) processes for clinical and commercial therapeutics.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-3'-O-T-Butyldimethylsilyl Uridine
CAS No.	81246-81-3
Molecular Formula	C32H40N2O8Si
Molecular Weight	608.76 g/mol
Synonyms	5'-O-DMT-3'-O-TBDMS-Uridine; 5'-O-(4,4'-Dimethoxytriphenylmethyl)-3'-O-(tert-butyldimethylsilyl)uridine; 3'-O-TBDMS-5'-O-DMT-Uridine; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-O-[(1,1-dimethylethyl)dimethylsilyl]-; DMTr-Uridine-TBDMS; Protected Uridine Phosphoramidite Precursor; NSC 339539
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-3'-O-T-Butyldimethylsilyl Uridine is manufactured under strict quality controls to ensure high purity and batch-to-batch consistency suitable for sensitive oligonucleotide synthesis. We provide comprehensive analytical data, including HPLC purity, NMR, and mass spectrometry confirmation. Certificates of Analysis (COA) detailing all specifications are available for every batch, supporting compliance with research and cGMP guidelines for therapeutic development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This material is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.