Description

8-Bromo-Adenosine-5'-Triphosphate, Sodium Salt is a chemically modified nucleotide analog where a bromine atom is substituted at the 8-position of the adenine ring. This modification is critical for its role as a versatile biochemical tool, particularly in structural biology and enzymology research. It is primarily utilized by researchers in pharmaceutical R&D, academic institutions, and biotechnology companies for studying enzyme mechanisms, nucleic acid interactions, and as a substrate analog in kinase assays.

Application

• Enzyme Kinetics & Mechanism Studies: Used as a substrate analog for ATP-dependent enzymes, including kinases, polymerases, and helicases, to probe active site architecture and catalytic pathways.
• Structural Biology (X-ray Crystallography): The bromine atom serves as a heavy atom for phasing in the determination of protein-nucleotide complex structures.
• Nucleic Acid Research: Acts as a chain terminator or modified substrate in studies of RNA/DNA synthesis and repair mechanisms.
• Biochemical Assay Development: Employed in high-throughput screening (HTS) assays to identify inhibitors of ATP-binding proteins.
• Photocrosslinking & Affinity Labeling: The bromo-modification can be leveraged for crosslinking experiments to map nucleotide-binding sites on proteins.
• Metabolic Pathway Analysis: Used as a tracer or probe in studies of cellular energy metabolism and nucleotide turnover.

Basic Information

Product Name	8-Bromo-Adenosine-5'-Triphosphate, Sodium Salt
CAS No.	81035-56-5
Molecular Formula	C10H13BrN5O13P3 · xNa+
Molecular Weight	Approx. 627.96 (Free acid) + sodium counterions
Synonyms	8-Br-ATP, Na Salt; 8-Bromo-ATP; 8-Bromoadenosine 5'-triphosphate trisodium salt; Adenosine-5'-triphosphate, 8-bromo-, sodium salt; 8-Br-adenosine-5'-triphosphate; Br8ATP; 8-Bromo-5'-ATP
EINECS	Contact for details

Quality Control

Our 8-Bromo-Adenosine-5'-Triphosphate, Sodium Salt is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% (ATP basis)
Water Content (KF)	≤ 5%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.