Description

2'-Deoxyadenylyl(3'-5')Thymidylyl(3'-5')Deoxyguanosylyl(3'-5')Thymidine is a defined-sequence oligonucleotide, specifically a tetramer, which serves as a critical building block and reference standard in nucleic acid research and development. Its precise structure makes it invaluable for studying DNA hybridization kinetics, enzyme mechanisms, and for calibrating analytical instruments. This high-purity biochemical is essential for pharmaceutical R&D, academic laboratories, and diagnostic kit manufacturers focused on molecular biology, antisense technology, and next-generation sequencing applications.

Application

• Oligonucleotide Synthesis Reference Standard: Used as a high-purity calibrant and control for quality assurance in solid-phase oligonucleotide synthesis.
• Enzymology Studies: Serves as a specific substrate or inhibitor for investigating the activity and kinetics of nucleases, polymerases, ligases, and other DNA-modifying enzymes.
• Diagnostic Probe Development: Acts as a core sequence or spacer in the design and validation of hybridization probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Antisense & Aptamer Research: Provides a defined short-sequence model for studying structure-activity relationships in the development of therapeutic oligonucleotides.
• Analytical Method Development: Employed as a known standard in HPLC, LC-MS, and capillary electrophoresis methods to establish system suitability and quantify impurities.
• Biophysical Studies: Used in research on DNA duplex stability, melting temperature (Tm) analysis, and structural characterization via NMR or X-ray crystallography.

Basic Information

Product Name	2'-Deoxyadenylyl(3'-5')Thymidylyl(3'-5')Deoxyguanosylyl(3'-5')Thymidine
CAS No.	80565-17-9
Molecular Formula	C39H48N15O23P3
Molecular Weight	1188.80 g/mol
Synonyms	d(ApTpGpT); d(A-T-G-T); Deoxyadenylyl-thymidylyl-deoxyguanylyl-thymidine; 5'-d(ATGT)-3'; Adenine, thymine, guanine, thymine tetramer; Oligodeoxyribonucleotide d(ATGT); DNA Tetramer d(ATGT); dAdTdGdT
EINECS	Contact for details

Quality Control

Every batch of our oligonucleotide is synthesized and purified under controlled conditions to meet exacting standards for research and development. Certificates of Analysis (COA) are provided, detailing purity assessed by advanced analytical techniques including HPLC and mass spectrometry. We ensure compliance with in-house specifications designed for critical biochemical applications, supporting traceability and reproducibility in your work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to theoretical mass
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Extinction Coefficient	Provided on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.