Description

3'-O-(3-(2-Nitro-4-Azidophenyl)Propionyl)Adenosine Triphosphate is a chemically modified, photoactivatable nucleotide analog designed for advanced biochemical research and molecular labeling. This compound is engineered to serve as a critical cross-linking agent and affinity probe in nucleic acid research, enabling the study of protein-nucleic acid interactions and enzyme mechanisms. It is primarily utilized by researchers and developers in biochemistry, molecular biology, and pharmaceutical discovery for applications requiring precise, light-induced covalent bonding.

Application

• Photoaffinity Labeling: Acts as a critical probe for identifying and characterizing ATP-binding sites in proteins and enzymes through UV-induced cross-linking.
• Enzyme Mechanism Studies: Used to investigate the active sites and catalytic mechanisms of kinases, polymerases, and other ATP-dependent enzymes.
• Nucleic Acid Research: Employed in the study of nucleic acid-protein interactions and the development of novel diagnostic assays.
• Bioconjugation and Probe Synthesis: Serves as a key reagent for synthesizing labeled nucleotides for advanced imaging and detection techniques.
• Drug Discovery Screening: Utilized in high-throughput screening platforms to identify and validate novel inhibitors targeting ATP-binding pockets.
• Structural Biology: Aids in mapping interaction interfaces for structural analysis via techniques like X-ray crystallography and cryo-EM.

Basic Information

Product Name	3'-O-(3-(2-Nitro-4-Azidophenyl)Propionyl)Adenosine Triphosphate
CAS No.	80220-04-8
Molecular Formula	C23H26N10O17P3
Molecular Weight	858.42 g/mol
Synonyms	3'-O-[3-(2-Nitro-4-azidophenyl)propionyl]ATP; 3'-(2-Nitro-4-azidobenzoyl)ATP; NAPP-ATP; 3'-O-(3-(2-Nitro-4-azidophenyl)propionyl)adenosine 5'-triphosphate; Photoactivatable ATP analog; 2-Nitro-4-azidophenylpropionyl-ATP; caged ATP derivative; ATP-arylazide photoaffinity label
EINECS	Contact for details

Quality Control

Every batch of our 3'-O-(3-(2-Nitro-4-Azidophenyl)Propionyl)Adenosine Triphosphate is manufactured and tested under strict quality protocols to ensure high purity, stability, and batch-to-batch consistency essential for sensitive research applications. Our quality assurance includes comprehensive analytical testing via HPLC, NMR, and MS. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and performance specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, aqueous buffer
Biological Activity	Functional in standard photo-crosslinking assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.