Description

Ethanol:Isopropanol 19:1, For Molecular Biology is a precisely formulated, high-purity solvent blend designed for critical life science applications. This specific 19:1 ratio of ethanol to isopropanol is optimized for procedures requiring controlled precipitation and dehydration, such as nucleic acid purification. It is an essential reagent for molecular biologists, diagnostic developers, and research laboratories where consistent performance and purity are non-negotiable. The product is manufactured and handled under stringent conditions to ensure it meets the exacting standards of modern molecular biology workflows.

Application

• Nucleic Acid Precipitation: Primary solvent for the precipitation of DNA and RNA from aqueous solutions, particularly in protocols for plasmid purification, cDNA synthesis, and next-generation sequencing library preparation.
• Cell and Tissue Fixation/Dehydration: Used in histological and cytological sample preparation for microscopy, providing effective dehydration while preserving cellular morphology.
• Laboratory Surface and Equipment Decontamination: Effective blend for disinfecting benches, pipettes, and non-autoclavable equipment in sterile work environments like laminar flow hoods and PCR workstations.
• Protein Crystallization: Serves as a precipitating agent in vapor diffusion and other crystallization methods for structural biology studies.
• Buffer and Reagent Preparation: A key component in the formulation of various molecular biology buffers and wash solutions, including those used in spin-column and magnetic bead-based kits.
• General Laboratory Solvent: Used for rinsing glassware, dissolving organic compounds, and as a carrier solvent in various analytical techniques.

Basic Information

Product Name	Ethanol:Isopropanol 19:1, For Molecular Biology
CAS No.	8013-53-4
Molecular Formula	Mixture (C₂H₆O / C₃H₈O)
Molecular Weight	Mixture
Synonyms	Ethanol-Isopropanol Blend (19:1); Ethyl Alcohol-Isopropyl Alcohol Mixture; DNA Grade Ethanol/IPA; Molecular Biology Grade Alcohol Blend; Precipitation Alcohol; 95% Ethanol / 5% Isopropanol; Decontamination Solution; Denatured Alcohol Blend
EINECS	Contact for details

Quality Control

This product is manufactured under strict quality control protocols to ensure it meets the stringent requirements for molecular biology applications. Each batch is tested to confirm identity, purity, and the absence of contaminants such as nucleases and protease. A Certificate of Analysis (COA) detailing specifications including assay by GC, water content, and absorbance profiles is provided with every shipment. Our quality system is designed to support compliance with ISO 9001 standards and good manufacturing practices (GMP) for reagent production.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, well-ventilated area at room temperature (15-25°C). Due to its highly volatile nature, keep away from heat, sparks, and open flames. Ensure containers are properly sealed when not in use to prevent evaporation and absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Clear, colorless liquid
Identification (IR)	Conforms
Assay (GC)	Ethanol: 94.0 - 96.0% (v/v); Isopropanol: 4.0 - 6.0% (v/v)
Water Content (KF)	≤ 0.5%
Absorbance (at 260 nm)	≤ 0.10
Absorbance (at 280 nm)	≤ 0.05
DNase Activity	Not detected
RNase Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.