Description

D(Gtaaaacgacggccagt) CAS NO 78809-73-1 is a synthetic oligonucleotide sequence, a critical building block for advanced molecular biology and genetic research. This high-purity compound is essential for applications requiring precise genetic manipulation and sequence-specific hybridization. It is primarily utilized by biotechnology firms, pharmaceutical R&D departments, and academic research institutions engaged in PCR, gene synthesis, and probe development.

Application

• Primer for Polymerase Chain Reaction (PCR): Used as a forward or reverse primer for amplifying specific DNA sequences in diagnostic and research applications.
• Gene Synthesis and Cloning: Serves as a fundamental component in the assembly of synthetic genes and the construction of plasmid vectors.
• Nucleic Acid Probe: Functions as a labeled or unlabeled probe for detecting complementary sequences in techniques like Southern blotting, Northern blotting, or FISH.
• Antisense Oligonucleotide Research: Investigated for its potential to modulate gene expression by binding to specific mRNA targets.
• Site-Directed Mutagenesis: Employed to introduce specific point mutations into a DNA sequence for functional studies.
• DNA Microarray Fabrication: Used as a spotted sequence on chips for high-throughput genomic analysis.
• Sequencing and Genotyping: Acts as a sequencing primer or as part of assay designs for genetic variant analysis.
• Therapeutic Nucleic Acid Development: Explored as a component in the development of oligonucleotide-based therapeutics.

Basic Information

Item	Detail
Product Name	D(Gtaaaacgacggccagt)
CAS No.	78809-73-1
Molecular Formula	C156H195N61O94P16 (estimated)
Molecular Weight	~ 4850 g/mol (estimated)
Synonyms	Deoxyguanylyl-(3'→5')-thymidylyl-(3'→5')-adenylyl-(3'→5')-adenylyl-(3'→5')-adenylyl-(3'→5')-adenylyl-(3'→5')-cytidylyl-(3'→5')-guanylyl-(3'→5')-adenylyl-(3'→5')-cytidylyl-(3'→5')-guanylyl-(3'→5')-guanylyl-(3'→5')-cytidylyl-(3'→5')-cytidylyl-(3'→5')-adenylyl-(3'→5')-guanylyl-(3'→5')-thymidine; Oligonucleotide Sequence d(GTAAAACGACGGCCAGT); DNA Oligo d(GTAAAACGACGGCCAGT); M13/pUC Forward Sequencing Primer (-21); -21 M13 Forward Primer; Universal Forward Primer
EINECS	Contact for details

Quality Control

Our oligonucleotides are manufactured under GMP-like conditions and undergo rigorous analytical testing to ensure sequence fidelity and purity. Each batch is controlled against stringent specifications for identity, purity, and concentration. Certificates of Analysis (COA) detailing HPLC purity, mass spectrometry (MS) confirmation, and endotoxin levels are available upon request, supporting applications in sensitive research and development.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation. For short-term use, store at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder or pellet
Identification (MS)	Consistent with theoretical mass
Purity (HPLC)	≥ 95% (Full Length Product)
Assay (UV)	≥ 85% (by OD260 units)
Extinction Coefficient	~ 168,800 L·mol-1·cm-1 (estimated)
Molecular Weight	Within ± 5 Da of theoretical
Endotoxin	< 10 EU/mg
Solubility	Soluble in TE buffer or nuclease-free water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.