Description

2-Iminobiotin-6-Aminohexyl-Sepharose 4B is an affinity chromatography medium designed for the specific purification of avidin, streptavidin, and related biotin-binding proteins. This specialized resin leverages the high-affinity interaction between the 2-iminobiotin ligand and its target proteins, enabling highly selective capture and elution under mild conditions. It is an essential tool for researchers and process developers in the biotechnology and pharmaceutical industries requiring high-purity protein isolates for diagnostics, therapeutics, and analytical applications.

Application

• Purification of Avidin and Streptavidin: Primary application for isolating these proteins from recombinant expression systems or natural sources.
• Biotin-Binding Protein Research: Used in biochemical research to study the structure, function, and kinetics of biotin-binding proteins.
• Diagnostic Kit Manufacturing: Critical for producing high-purity streptavidin conjugates used in ELISA, lateral flow assays, and other immunoassays.
• Therapeutic Protein Downstream Processing: Employed in the purification process for biotinylated therapeutic antibodies or fusion proteins in GMP environments.
• Affinity Tag System Development: Serves as a capture matrix in systems utilizing a biotin or strep-tag for recombinant protein purification.
• Sample Preparation for Structural Biology: Provides highly pure protein samples suitable for techniques like X-ray crystallography or cryo-EM.

Basic Information

Product Name	2-Iminobiotin-6-Aminohexyl-Sepharose 4B
CAS No.	78690-04-7
Molecular Formula	N/A (Chromatography Medium)
Molecular Weight	N/A
Synonyms	2-Iminobiotin-AH-Sepharose 4B; 2-Iminobiotinyl-6-aminohexyl-Sepharose 4B; Iminobiotin Agarose; 6-(2-Iminobiotinylamino)hexyl-Sepharose 4B; Avidin Purification Resin; Streptavidin Affinity Gel; Iminobiotin Affinity Medium; Sepharose 4B-Iminobiotin
EINECS	Contact for details

Quality Control

Our affinity chromatography media are produced under a rigorous quality management system. Each lot is tested for key performance parameters including ligand density, binding capacity, flow characteristics, and absence of microbial contamination to ensure consistent and reproducible purification performance. Certificates of Analysis (COA) detailing these specifications are provided with every shipment. The product is suitable for use in research and process development under ISO 9001 standards.

Storage

Preserve in a tightly closed container, protected from light. Store the supplied suspension at 2-8°C in 20% ethanol. Do not freeze. Under these conditions, the product is stable for the period indicated on the label.

Specification

Item	Specification
Matrix	Cross-linked 4% Agarose (Sepharose 4B)
Ligand	2-Iminobiotin
Spacer Arm	6-Aminohexyl
Binding Capacity	> 2 mg avidin/mL settled gel
Particle Size	45-165 μm
Storage Solution	20% Ethanol
Microbial Bioburden	To specification

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.