Description

Inosine 5'-(3-Thiotriphosphate) is a chemically modified nucleotide analog where one of the non-bridging oxygen atoms in the triphosphate chain is replaced by a sulfur atom. This thiophosphate modification makes it a crucial tool in biochemical research, particularly as a substrate for RNA polymerases and as a non-hydrolyzable or slowly hydrolyzable analog of ATP/GTP in enzymatic studies. It is essential for researchers in molecular biology, enzymology, and structural biology who require precise probes to study nucleotide-dependent processes, kinase activity, and nucleic acid synthesis. The compound is supplied as a high-purity material suitable for sensitive experimental applications.

Application

• Enzymology Research: Used as a substrate analog to study the mechanisms of ATP/GTP-dependent enzymes, including kinases, helicases, and polymerases, due to its resistance to hydrolysis.
• RNA Synthesis Studies: Serves as a substrate for RNA polymerases in in vitro transcription to produce thiophosphate-containing RNA for structural and functional analysis.
• Biophysical Probes: Employed in X-ray crystallography and other structural biology techniques to trap and visualize intermediate states of nucleotide-binding proteins.
• Signal Transduction Research: Acts as a non-hydrolyzable ligand to investigate G-protein and other GTPase-mediated signaling pathways.
• Pharmaceutical Development: Utilized in high-throughput screening assays to identify and characterize inhibitors of nucleotide-binding enzymes as potential drug targets.
• Diagnostic Assay Development: Incorporated into enzymatic assays for the detection and quantification of specific enzymatic activities in clinical and research settings.

Basic Information

Product Name	Inosine 5'-(3-Thiotriphosphate)
CAS No.	77065-87-3
Molecular Formula	C10H15N4O13P3S
Molecular Weight	524.22 g/mol
Synonyms	Inosine 5'-(γ-Thiotriphosphate); ITPγS; 5'-ITP-γ-S; Inosine 5'-triphosphate-γ-thio; Guanosine 5'-[γ-thio]triphosphate analog (inosine base); 9-β-D-Ribofuranosylhypoxanthine 5'-(3-thiotriphosphate); 5'-O-[Hydroxy([hydroxy(phosphonooxy)phosphoryl]thio)phosphoryl]inosine
EINECS	Contact for details

Quality Control

Our Inosine 5'-(3-Thiotriphosphate) is manufactured under strict quality controls to ensure high purity and batch-to-batch consistency suitable for research applications. Each lot is analyzed to confirm identity, purity, and the absence of critical impurities such as nucleoside monophosphates and diphosphates. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications as determined by HPLC, NMR, and UV-Vis spectroscopy. We support compliance with relevant quality frameworks for research chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, it is recommended to keep the material desiccated and under inert atmosphere to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
UV-Vis Analysis	Conforms to characteristic spectrum
Biological Activity	Suitable for enzyme studies

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.