Description

5''-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-3''-Thymidylic Acid Bis(2-Cyanoethyl) Ester is a sophisticated, protected nucleoside derivative primarily used as a critical building block in oligonucleotide synthesis. This compound matters because it enables the precise, automated assembly of DNA and RNA sequences by protecting the reactive hydroxyl groups during the coupling process. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries who are developing advanced therapeutics, probes, and molecular tools.

Application

• Oligonucleotide Synthesis: Serves as a key phosphoramidite precursor for the solid-phase synthesis of DNA and RNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides for therapeutic applications.
• PCR Primer & Probe Manufacturing: Essential for creating high-purity primers and fluorescent probes used in genetic testing and diagnostics.
• siRNA and miRNA Synthesis: A critical raw material in the manufacture of small interfering RNA (siRNA) and microRNA (miRNA) for gene silencing studies and therapies.
• Research Reagents: Employed in academic and industrial R&D for studying nucleic acid chemistry and developing novel molecular biology techniques.
• Diagnostic Kit Components: Integrated into the production of components for kits used in pathogen detection, genetic screening, and personalized medicine.

Basic Information

Product Name	5''-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-3''-Thymidylic Acid Bis(2-Cyanoethyl) Ester
CAS No.	76759-76-7
Molecular Formula	C38H39N3O9
Molecular Weight	681.74 g/mol
Synonyms	5'-O-DMT-2'-Deoxythymidine-3'-(2-Cyanoethyl N,N-Diisopropylphosphoramidite); Thymidine 5'-O-(4,4'-Dimethoxytrityl)-3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidite); DMT-dT-CE Phosphoramidite; dT Phosphoramidite (DMT protected); 2'-Deoxythymidine-5'-O-(4,4'-dimethoxytrityl)-3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidite); 5'-O-(4,4'-Dimethoxytrityl)thymidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); dT-CE Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5''-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-3''-Thymidylic Acid Bis(2-Cyanoethyl) Ester is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is analyzed using advanced techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and stability. Certificates of Analysis (COA) with full analytical data are provided to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a recommended temperature of -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its moisture-sensitive and light-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.