Description

Thymidyl 3'-(4-Nitrophenyl)Phosphorothioate is a specialized nucleotide analog featuring a phosphorothioate linkage and a chromogenic nitrophenyl leaving group. This compound is a critical reagent in enzymatic and biochemical research, particularly for studying the kinetics and mechanisms of nucleases, polymerases, and other phosphotransferase enzymes. It is essential for researchers and manufacturers in the fields of molecular biology, enzymology, and pharmaceutical development who require high-purity, well-characterized substrates for assay development and mechanistic studies.

Application

• Nuclease Activity Assays: Used as a chromogenic substrate to measure the activity of restriction endonucleases, exonucleases, and ribonucleases.
• Enzyme Kinetics Studies: Serves as a model substrate for investigating the catalytic mechanism and inhibition of phosphodiesterases and polymerases.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential therapeutic inhibitors of nucleic acid-processing enzymes.
• Biochemical Research: A key tool for studying DNA/RNA repair pathways, apoptosis, and other cellular processes involving nucleic acid degradation.
• Diagnostic Kit Development: Incorporated into kits for the detection and quantification of specific nuclease activities in clinical or research samples.

Basic Information

Product Name	Thymidyl 3'-(4-Nitrophenyl)Phosphorothioate
CAS No.	76756-12-2
Molecular Formula	C16H18N3O9PS
Molecular Weight	459.37 g/mol
Synonyms	3'-Thymidylyl (4-nitrophenyl) phosphorothioate; p-Nitrophenyl 5'-O-(thymidin-3'-yl) phosphorothioate; Thymidine 3'-(p-nitrophenyl) phosphorothioate; Tp(s)(pNph); Nucleotide phosphorothioate substrate; CAS 76756-12-2
EINECS	Contact for details

Quality Control

We supply Thymidyl 3'-(4-Nitrophenyl)Phosphorothioate to high-purity standards suitable for sensitive biochemical applications. Every batch undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided to ensure traceability and support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.