Description

Guanosine-5'-o-(3-Thio-(Acetylaminoethyl)-1-Naphthylamine-5-Sulfonic Acid)Triphosphate is a specialized, chemically modified nucleotide analog designed for advanced biochemical and diagnostic applications. This compound is critical for researchers and developers requiring high-fidelity labeling and detection in complex biological systems. It is primarily utilized in the life sciences sector, particularly in molecular biology, next-generation sequencing (NGS) workflows, and diagnostic assay development.

Application

• Next-Generation Sequencing (NGS): Used as a modified nucleotide triphosphate for sequencing-by-synthesis platforms to enable novel detection chemistries.
• Diagnostic Assay Development: Serves as a key component in the synthesis of probes for fluorescence-based or chemiluminescent diagnostic tests.
• Enzymatic Labeling: Acts as a substrate for polymerases and other enzymes in enzymatic incorporation assays for nucleic acid labeling.
• Bioconjugation & Probe Synthesis: The reactive thio and sulfonic acid groups facilitate conjugation to proteins, surfaces, or other molecules for creating detection probes.
• Cellular Imaging Studies: Can be incorporated into nucleic acids to study cellular processes via advanced microscopy techniques.
• Drug Discovery Research: Employed in high-throughput screening assays targeting nucleotide-binding proteins or enzymes.

Basic Information

Product Name	Guanosine-5'-o-(3-Thio-(Acetylaminoethyl)-1-Naphthylamine-5-Sulfonic Acid)Triphosphate
CAS No.	76709-18-7
Molecular Formula	C₂₄H₂₉N₈O₁₇P₃S₂
Molecular Weight	874.59 g/mol
Synonyms	5'-Guanosine-O-(3-thio(acetylaminoethyl)-1-naphthylamine-5-sulfonic acid) triphosphate; Thio-ANSA-GTP; Modified GTP analog; ANSA-GTP; GTP-ε-ANSA; 5'-[γ-[3-[(2-Acetamidoethyl)thio]-1-naphthylamino]-5-sulfonato]triphosphate guanosine; N⁶-(3-[(2-Acetamidoethyl)thio]-1-naphthylamino-5-sulfonic acid)-GTP; GTP-γ-ANSA
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and functional performance. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for assay, impurity profile, and bioburden where applicable. We support compliance with ISO 13485 standards for diagnostic component manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in aqueous buffer, DMSO
Functional Activity	Tested in enzymatic incorporation assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.