Description

Valyl-Phenylalanyl-Lysine-4-Nitroanilide is a synthetic tripeptide substrate designed for enzymatic activity studies. This compound is a critical tool for researchers and quality control laboratories requiring precise measurement of specific protease activity. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic assay manufacturing where accurate enzyme kinetics are paramount.

Application

• Protease Enzyme Assays: Acts as a chromogenic substrate for the kinetic study and quantification of specific serine or cysteine proteases.
• Biochemical Research: Used in academic and industrial R&D to investigate enzyme mechanisms, inhibition, and substrate specificity.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic enzyme inhibitors.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic tests that measure protease levels in biological samples.
• Quality Control (QC) Testing: Used to standardize and validate the activity of enzyme batches in biopharmaceutical production.

Basic Information

Product Name	Valyl-Phenylalanyl-Lysine-4-Nitroanilide
CAS No.	76626-36-3
Molecular Formula	C30H42N8O6
Molecular Weight	610.71 g/mol
Synonyms	Val-Phe-Lys-pNA; VFK-pNA; H-Val-Phe-Lys-pNA; Valylphenylalanyllisine 4-nitroanilide; Substrate for Plasmin; Plasmin Substrate VFK-pNA; Chromogenic Plasmin Substrate; 4-Nitroanilide of Val-Phe-Lys
EINECS	Contact for details

Quality Control

Our Valyl-Phenylalanyl-Lysine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.