Description

Pth-Histidine CAS NO 5835-68-7 is a protected derivative of the essential amino acid L-Histidine, where the α-amino group is blocked by a phthaloyl (Pth) protecting group. This modification is critical for enabling selective peptide synthesis and advanced biochemical research where specific functional group reactivity must be controlled. It is an indispensable building block for pharmaceutical R&D, particularly in the development of peptide-based therapeutics, diagnostic agents, and as a key intermediate in organic synthesis for the life sciences.

Application

• Peptide Synthesis: Serves as a crucial protected intermediate in solid-phase and solution-phase peptide synthesis (SPPS) to prevent unwanted side reactions during coupling steps.
• Pharmaceutical Intermediates: Used in the research and production of active pharmaceutical ingredients (APIs), especially those containing histidine residues.
• Biochemical Research: Employed in enzymatic studies, protein engineering, and as a standard or reagent in analytical method development.
• Protecting Group Chemistry: Acts as a model compound for studying the introduction and removal (e.g., with hydrazine) of the phthaloyl protecting group in synthetic organic chemistry.
• Reference Standard: Utilized as a high-purity chemical reference standard in quality control laboratories for HPLC, LC-MS, or NMR analysis.

Basic Information

Product Name	Pth-Histidine
CAS No.	5835-68-7
Molecular Formula	C14H13N3O4
Molecular Weight	287.27 g/mol
Synonyms	N-Phthaloyl-L-histidine; Phthaloylhistidine; L-Histidine, N-phthaloyl-; (S)-2-(1H-Imidazol-4-ylmethyl)-4-phthalimidobutanoic Acid; Nα-Phthaloyl-L-histidine; PTH-His-OH; N-Pth-L-His; Fmoc-His(Trt)-OH precursor intermediate
EINECS	Contact for details

Quality Control

Our Pth-Histidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, optical rotation measurement, and identity confirmation via IR and NMR. We provide full traceability and detailed Certificates of Analysis (COA) to support your regulatory and research requirements. Specifications can be aligned with research-grade or GMP-grade standards upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccants under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Optical Rotation [α]	Contact for details
Water Content (KF)	≤1.0%
Heavy Metals	≤20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.