Description

Phenylthiohydantoin-Threonine (PTH-Threonine) is a specialized derivative of the amino acid threonine, formed via the Edman degradation process. This compound is a critical analytical reagent for the precise sequencing and identification of proteins and peptides. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors who require high-purity standards for their analytical workflows.

Application

• Protein Sequencing: As a key derivative in the Edman degradation method for stepwise N-terminal amino acid analysis.
• Peptide Mapping & Characterization: Used in the identification and structural elucidation of synthetic and natural peptides.
• Biopharmaceutical Quality Control: Employed in the analysis of recombinant proteins, monoclonal antibodies, and other therapeutic biologics to confirm amino acid composition.
• Proteomics Research: A valuable tool in academic and industrial research for studying protein structure, function, and post-translational modifications.
• Amino Acid Analysis: Serves as a reference standard in chromatographic methods (HPLC, UPLC) for the quantification of threonine and related derivatives.
• Diagnostic Reagent Manufacturing: Used in the production of kits and reagents for clinical diagnostics and biochemical testing.

Basic Information

Product Name	Phenylthiohydantoin-Threonine
CAS No.	5789-21-9
Molecular Formula	C11H12N2O3S
Molecular Weight	252.29 g/mol
Synonyms	PTH-Threonine; 3-[(Phenylamino)thioxomethyl]amino-2-butanone; 2-Thiohydantoin, 5-(1-hydroxyethyl)-3-phenyl-; Edman Degradation Product of Threonine; 5-(1-Hydroxyethyl)-3-phenyl-2-thiohydantoin; Threonine Phenylthiohydantoin; PTH-Thr
EINECS	227-324-5

Quality Control

Our Phenylthiohydantoin-Threonine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Related Substances (HPLC)	Individual impurity ≤ 0.5%; Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.