Description

5'-O-(4,4'-Dimethoxytrityl)-N6-Benzoyl-2'-Deoxyadenosine-3'-O-Succinic Acid CAS NO 74405-42-8 is a critical, high-purity phosphoramidite monomer derivative essential for the solid-phase synthesis of oligonucleotides. This compound is specifically designed to introduce the protected deoxyadenosine nucleotide during automated DNA/RNA synthesis, ensuring high coupling efficiency and sequence fidelity. It is a fundamental building block for research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing therapeutics, primers, and probes.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated solid-phase synthesis of DNA strands.
• Therapeutic Development: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical research and drug discovery.
• Diagnostic Probes & Primers: Manufacture of labeled and unlabeled probes and primers used in PCR, qPCR, and next-generation sequencing (NGS) applications.
• Research Reagents: Synthesis of custom oligonucleotides for academic and industrial research in genomics, molecular biology, and biochemistry.
• CRISPR/Cas9 Components: Synthesis of guide RNAs (gRNAs) and other components for gene-editing technologies.
• Bio-conjugation & Labeling: The succinyl linker enables further functionalization and conjugation of oligonucleotides to surfaces, fluorophores, or other molecules.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N6-Benzoyl-2'-Deoxyadenosine-3'-O-Succinic Acid
CAS No.	74405-42-8
Molecular Formula	C48H43N5O11
Molecular Weight	865.88 g/mol
Synonyms	5'-DMT-N6-Bz-dA-3'-succinate; DMTr-dA(Bz)-succinate; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine-3'-O-succinate; dA(Bz) DMT Succinyl CPG Precursor; 2'-Deoxy-N6-benzoyl-5'-O-(4,4'-dimethoxytrityl)adenosine-3'-O-succinic Acid; Protected Deoxyadenosine-3'-succinate; DMT-dA(Bz)-Su; 74405-42-8
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N6-Benzoyl-2'-Deoxyadenosine-3'-O-Succinic Acid is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high purity and correct structural identity. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, moisture content, and identity confirmation. Production can adhere to GMP guidelines for therapeutic-grade applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) to protect this hygroscopic and moisture-sensitive compound. For long-term storage, keep under an inert atmosphere (argon or nitrogen) to prevent degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.