Description

2'-Deoxy-2'-Azidoadenosine Triphosphate is a chemically modified nucleotide analog where the 2'-hydroxyl group of the ribose sugar is replaced by an azido (-N3) functionality, while retaining the high-energy triphosphate moiety. This unique structure makes it a critical reagent for click chemistry applications, particularly in the site-specific labeling and functionalization of nucleic acids. It is primarily utilized by researchers and developers in the fields of molecular biology, biochemistry, and pharmaceutical development for creating advanced probes and studying nucleic acid metabolism and interactions.

Application

• Click Chemistry Substrate: Acts as a key building block for copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions to label DNA or RNA.
• Nucleic Acid Labeling & Functionalization: Enables the site-specific incorporation of fluorescent dyes, biotin, or other tags into oligonucleotides for detection and purification.
• Enzymatic Studies: Used as a substrate or inhibitor to study the activity and specificity of DNA/RNA polymerases, kinases, and other nucleotide-processing enzymes.
• Probe Development: Essential for synthesizing cross-linking probes or affinity probes to investigate nucleic acid-protein interactions.
• Drug Discovery Research: Serves as a tool compound in the development of antiviral or anticancer agents that target nucleotide metabolism pathways.
• Next-Generation Sequencing (NGS): Potential use in the development of novel sequencing chemistries or modified nucleotides for specialized NGS applications.

Basic Information

Product Name	2'-Deoxy-2'-Azidoadenosine Triphosphate
CAS No.	73449-06-6
Molecular Formula	C10H14N8O12P3
Molecular Weight	555.16 g/mol
Synonyms	2'-Azido-2'-deoxyadenosine-5'-triphosphate; 2'-N3-dATP; 2'-Azido-dATP; 2'-Deoxy-2'-azidoadenosine 5'-triphosphate; Azido-dATP; 2'-Azido-2'-deoxyATP; Adenosine, 2'-azido-2'-deoxy-, 5'-(tetrahydrogen triphosphate)
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-2'-Azidoadenosine Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to rigorous internal quality standards to guarantee product integrity, and specifications can be tailored to meet specific research-grade or custom synthesis requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, desiccate and keep frozen.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.