Description

X-Gal is a high-purity chromogenic substrate widely used in molecular biology and biochemistry for the detection of β-galactosidase activity. This compound is critical for blue-white screening in recombinant DNA technology, enabling the rapid and visual identification of successful cloning events. It is an essential reagent for researchers and manufacturers in biotechnology, pharmaceutical development, and diagnostic kit production who require reliable and sensitive detection methods.

Application

• Molecular Biology Research: Primary substrate for blue-white screening in plasmid and phage vector systems to identify recombinant bacterial colonies.
• Gene Expression Studies: Used as an indicator in assays to monitor and quantify β-galactosidase reporter gene (lacZ) expression.
• Diagnostic Development: A key component in the formulation of diagnostic kits and test strips for enzymatic detection.
• Cell Biology & Histochemistry: Employed in staining protocols to visualize β-galactosidase activity in fixed cells and tissue sections.
• High-Throughput Screening (HTS): Utilized in automated screening platforms for drug discovery and genetic research.
• Microbiology: Used in specialized media for the identification and differentiation of bacterial strains based on enzymatic activity.

Basic Information

Product Name	X-Gal
CAS No.	72122-63-5
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside; BCIG; 5-Bromo-4-chloro-3-indolyl β-D-galactoside; X-β-Gal; Galactoside indoxyl; BCI-GAL; 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our X-Gal is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by spectroscopic methods, and testing for suitability in enzymatic assays. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Approx. 210-215°C (dec.)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Functional Test	Suitable for blue-white screening

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.