Description

Guanosine 5'-o-(2-Thiodiphosphate) is a chemically modified nucleotide analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the diphosphate moiety. This compound is a critical biochemical tool used as a substrate analog to study the mechanisms of GTP-binding proteins (G-proteins), kinases, and other nucleotide-dependent enzymes. It is essential for researchers and developers in the fields of enzymology, molecular pharmacology, and signal transduction research. The product is supplied as a high-purity biochemical reagent to ensure reliable and reproducible experimental results.

Application

• Enzymology Research: Used as a non-hydrolyzable or slowly hydrolyzable GTP analog to probe the catalytic mechanisms of GTPases, including Ras superfamily proteins, translation factors, and signal transduction G-proteins.
• Signal Transduction Studies: Serves as a crucial tool for investigating nucleotide binding, hydrolysis, and exchange in G-protein coupled receptor (GPCR) pathways and other cellular signaling networks.
• Kinase & ATPase Assays: Employed as a substrate or inhibitor in enzymatic assays to study the specificity and kinetics of various nucleoside triphosphate-utilizing enzymes.
• Structural Biology: Used in X-ray crystallography and NMR studies to obtain high-resolution structures of enzyme-nucleotide complexes, providing insights into active site architecture.
• Drug Discovery & Screening: Acts as a key reagent in high-throughput screening (HTS) assays designed to identify novel modulators of nucleotide-binding proteins, which are important therapeutic targets.
• Biochemical Reagent Production: Incorporated into diagnostic kits and specialized reagent formulations for research and clinical testing applications.

Basic Information

Product Name	Guanosine 5'-o-(2-Thiodiphosphate)
CAS No.	71376-97-1
Molecular Formula	C10H16N5O13P3S
Molecular Weight	555.22 g/mol
Synonyms	Guanosine 5'-[β-thio]diphosphate; GDPβS; Guanosine-5'-diphosphorothioate; 5'-GDP-β-S; Guanosine 5'-(2-thiodiphosphate); 2'-Deoxyguanosine-5'-O-[1-thiodiphosphate]; GMPPCP (Note: sometimes used interchangeably, though typically refers to a different analog); Non-hydrolyzable GTP analog
EINECS	Contact for details

Quality Control

Our Guanosine 5'-o-(2-Thiodiphosphate) is manufactured under controlled conditions to meet the stringent requirements of biochemical research. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment and NMR for structural confirmation. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request. We adhere to a quality management system to ensure batch-to-batch consistency and reliability for your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.