Description

Dna, D(T-Sp-C-G-Sp-T-Sp-C-G-Sp-T-Sp-T-Sp-T-Sp-T-Sp-G-Sp-A-Sp-C-G-Sp-T-Sp-T-Sp-T-Sp-T-Sp-G-Sp-T-Sp-C-G-Sp-T-Sp-T) (9Ci) is a defined, high-purity oligonucleotide sequence containing phosphorothioate (Sp) backbone modifications, which are critical for enhancing nuclease resistance and stability in biological applications. This chemically modified DNA sequence is essential for advanced research and development in molecular biology, particularly for studies requiring precise genetic targeting and manipulation. It serves as a vital tool for scientists and developers in the pharmaceutical, biotechnology, and diagnostic sectors, where reliable and specific oligonucleotide probes or primers are required.

Application

• Antisense Oligonucleotide (ASO) Research: Used as a model or therapeutic candidate sequence in studies investigating gene silencing and modulation of protein expression.
• PCR & Molecular Diagnostics: Serves as a specialized primer or probe with enhanced stability for sensitive and specific detection of genetic markers.
• Nucleic Acid Therapeutics Development: A key intermediate in the R&D pipeline for novel drugs targeting genetic diseases, cancers, and viral infections.
• Biophysical & Structural Studies: Employed to investigate the properties of phosphorothioate-modified DNA, including duplex stability, protein interactions, and cellular uptake.
• Gene Editing & Functional Genomics: Utilized as a guide or template in advanced techniques like CRISPR-Cas systems that benefit from nuclease-resistant oligonucleotides.
• Diagnostic Kit Manufacturing: Incorporated as a critical component in commercial diagnostic assays and kits that require high specificity and batch-to-batch consistency.

Basic Information

Product Name	Dna, D(T-Sp-C-G-Sp-T-Sp-C-G-Sp-T-Sp-T-Sp-T-Sp-T-Sp-G-Sp-A-Sp-C-G-Sp-T-Sp-T-Sp-T-Sp-T-Sp-G-Sp-T-Sp-C-G-Sp-T-Sp-T) (9Ci)
CAS No.	665058-78-6
Molecular Formula	C235H293N79O131P27S9
Molecular Weight	~7240.5 g/mol (Theoretical Average)
Synonyms	Phosphorothioate-modified Oligonucleotide (27-mer); PS-oligonucleotide; DNA Oligomer with 9 Phosphorothioate Linkages; Antisense Oligo Sequence; d(TpsCpsGpsTpsCpsGpsTpsTpsTpsTpsGpsApsCpsGpsTpsTpsTpsTpsGpsTpsCpsGpsTpsT); Thioate DNA; nuclease-resistant DNA; Therapeutic Oligonucleotide
EINECS	Contact for details

Quality Control

Every batch of our oligonucleotides is manufactured under strict quality management systems. Purity and identity are confirmed through a battery of analytical techniques including HPLC, Mass Spectrometry (MS), and UV spectroscopy. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, concentration, and modification analysis. Our production adheres to GMP-like guidelines suitable for research and pre-clinical development, ensuring reliability and traceability for critical applications.

Storage

Preserve in a tightly closed container, protected from light. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated at -20°C. For short-term use, store at 2-8°C. Allow the vial to reach room temperature before opening to minimize moisture condensation. Solutions should be prepared with nuclease-free water or buffer and used promptly or stored frozen in aliquots.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Full-length product)
Assay (UV)	≥ 85% (by weight)
Modification Analysis (P-S ratio)	≥ 9 phosphorothioate linkages confirmed
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.