Description

Tris Borate EDTA Buffer, 10X, DNase, RNase and Protease Free, pH 8.3, for Molecular Biology is a ready-to-use, concentrated electrophoresis buffer solution formulated for high-resolution nucleic acid separation. This product is critical for ensuring the integrity and accuracy of sensitive molecular biology experiments, as it is rigorously processed to eliminate contaminating nucleases and proteases. It is an essential reagent for research laboratories, diagnostic kit manufacturers, and biopharmaceutical companies engaged in DNA/RNA analysis, genotyping, and sequencing workflows.

Application

• Agarose Gel Electrophoresis: Primary buffer for the separation and analysis of DNA and RNA fragments.
• DNA Sequencing: Used in capillary and slab gel electrophoresis systems for Sanger sequencing applications.
• Genotyping & PCR Product Analysis: Provides consistent migration for accurate sizing of PCR amplicons and restriction fragments.
• Northern & Southern Blotting: Serves as the running buffer in blotting procedures for nucleic acid detection.
• Quality Control in NGS: Employed in library preparation QC steps to assess fragment size distribution.
• Diagnostic Assay Manufacturing: A key component in the production of kits for genetic testing and molecular diagnostics.
• Forensic Analysis: Used in DNA fingerprinting and other forensic biology techniques requiring nuclease-free conditions.

Basic Information

Product Name	Tris Borate EDTA Buffer, 10X, DNase, RNase and Protease Free, pH 8.3, for Molecular Biology
CAS No.	610769-35-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	TBE Buffer, 10X; Tris-Borate-EDTA Buffer; TBE Running Buffer; Electrophoresis Buffer, 10X TBE; Nucleic Acid Electrophoresis Buffer; Nuclease-Free TBE Buffer; Tris Borate EDTA, pH 8.3; Molecular Biology Grade TBE
EINECS	Contact for details

Quality Control

This molecular biology grade buffer is manufactured under strict quality control protocols to guarantee performance. Every batch is tested to confirm the absence of DNase, RNase, and protease activity. Certificates of Analysis (COA) detailing pH, conductivity, and nuclease/protease testing are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C). For long-term stability, avoid repeated freeze-thaw cycles if aliquoted.

Specification

Item	Specification
Appearance	Clear, colorless solution
Concentration	10X
pH (at 25°C)	8.3 ± 0.2
Conductivity	Consistent with standard
DNase Activity	Not detected
RNase Activity	Not detected
Protease Activity	Not detected
Endotoxin	< 0.25 EU/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.