Description

Deoxyribonucleic Acid, Mammalian Virus From Simian Virus 40 is a high-purity, viral genomic DNA preparation derived from the Simian Virus 40 (SV40). This product is essential for molecular biology research, providing a well-characterized, circular double-stranded DNA template for a wide range of experimental applications. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical R&D departments for studies in virology, gene regulation, DNA replication, and as a critical reagent in transfection and cloning protocols.

Application

• Molecular Biology Research: Serves as a standard template for studying DNA replication mechanisms, transcription, and chromatin structure.
• Virology Studies: Used as a model system for investigating polyomavirus biology, viral oncogenesis, and host-cell interactions.
• Transfection Control: Acts as a positive control in transfection efficiency experiments for both transient and stable expression studies.
• Cloning & Vector Development: Provides source DNA for the construction of viral vectors, shuttle vectors, and other recombinant DNA molecules.
• Enzyme Activity Assays: Utilized as a substrate for testing the activity of restriction enzymes, ligases, polymerases, and topoisomerases.
• Gene Expression Analysis: Employed in promoter analysis and the study of enhancer elements using reporter gene constructs.
• Reference Standard: Functions as a quantitative and qualitative standard in gel electrophoresis, Southern blotting, and PCR applications.
• Biopharmaceutical Development: Used in early-stage R&D for vaccine development and gene therapy research targeting viral models.

Basic Information

Product Name	Deoxyribonucleic Acid, Mammalian Virus From Simian Virus 40
CAS No.	67801-85-8
Molecular Formula	N/A (Biopolymer)
Molecular Weight	~3.2 x 106 Da (for SV40 genome)
Synonyms	SV40 DNA; Simian Virus 40 DNA; Polyomavirus SV40 Genomic DNA; Viral DNA (SV40); SV40 Genome; Closed Circular SV40 DNA; Form I SV40 DNA; Supercoiled SV40 DNA; Mammalian Virus DNA
EINECS	Contact for details

Quality Control

Our SV40 DNA is produced and tested under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous quality testing, including analysis for purity, concentration, and structural integrity. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications such as A260/A280 ratio, gel electrophoresis profile, and confirmation of the supercoiled (Form I) conformation. Production follows relevant quality guidelines suitable for research use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquotting upon receipt is recommended for frequent use.

Specification

Item	Specification
Appearance	Clear, colorless to slightly opalescent solution
Concentration	As per COA (typically 0.5 - 1.0 mg/mL)
Purity (A260/A280)	1.8 - 2.0
Form	Predominantly supercoiled (Form I)
Identification (Gel Electrophoresis)	Single band corresponding to ~5.2 kb
Endotoxin Level	< 1.0 EU/µg DNA
Biological Activity (Transfection)	Functional in standard mammalian cell lines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.