Description

Blue Sepharose Cl-6B is a beaded affinity chromatography medium designed for the purification of proteins and enzymes, specifically those that bind nucleotide cofactors. This product is crucial for researchers and process developers requiring high-resolution separation and purification in both laboratory and pilot-scale bioprocessing. It is essential for the downstream purification workflows in the biotechnology, pharmaceutical, and life science research industries.

Application

• Affinity purification of dehydrogenases, kinases, and other nucleotide-binding proteins.
• Laboratory-scale process development and optimization for protein purification protocols.
• Pilot-scale purification of therapeutic enzymes and recombinant proteins in biomanufacturing.
• Removal of specific contaminant proteins from complex biological mixtures.
• Immobilization of ligands for studying protein-ligand interactions.
• Teaching and research applications in academic biochemistry and molecular biology laboratories.

Basic Information

Item	Details
Product Name	Blue Sepharose Cl-6B
CAS No.	67115-58-6
Molecular Formula	N/A (Cross-linked beaded agarose matrix with immobilized Cibacron Blue F3G-A dye)
Molecular Weight	N/A (Macromolecular resin)
Synonyms	Cibacron Blue F3G-A Sepharose CL-6B; Blue Sepharose 6 Fast Flow; Cibacron Blue 3GA-Agarose; Reactive Blue 2 Sepharose CL-6B; Affi-Gel Blue; Dye-ligand chromatography medium; Blue Dextran Sepharose CL-6B; Cibacron Blue F3GA-agarose
EINECS	Contact for details

Quality Control

Our Blue Sepharose Cl-6B is manufactured under a strict quality management system. Each batch undergoes rigorous testing for key performance parameters including ligand density, binding capacity, flow properties, and absence of microbial contamination to ensure consistent and reproducible chromatographic performance. Certificates of Analysis (COA) detailing these specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in 20% ethanol as a preservative. Do not freeze. Keep away from strong alkali and organic solvents as indicated by its chemical properties.

Specification

Item	Specification
Matrix	Cross-linked 6% agarose beads
Ligand	Cibacron Blue F3G-A
Particle Size	45-165 μm
Binding Capacity	≥ 10 mg albumin/ml gel
Maximum Flow Rate	150 cm/h
pH Stability	3 - 13 (long term); 2 - 14 (short term)
Chemical Stability	Stable in all commonly used aqueous buffers, detergents, and 6M guanidine HCl
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.