Description

Poly(D(A-C).D(G-T)) CAS NO 64816-49-5 is a synthetic, double-stranded alternating copolymer of deoxyadenosine-deoxycytidine and deoxyguanosine-deoxythymidine, widely utilized as a critical reference standard and model system in nucleic acid research. This compound is essential for studying DNA structure, stability, and interactions with proteins, drugs, and other biomolecules under controlled conditions. It is primarily needed by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in molecular biology, biophysical analysis, and therapeutic development.

Application

• Biophysical Research: Used as a model duplex for studying DNA melting temperature (Tm), hybridization kinetics, and helix-coil transitions.
• Drug Discovery Screening: Serves as a target substrate for high-throughput screening of potential small molecules, antibiotics, or chemotherapeutic agents that bind to or intercalate with DNA.
• Enzyme Activity Assays: Acts as a standardized substrate for characterizing the activity, specificity, and kinetics of nucleases, polymerases, ligases, and DNA-modifying enzymes.
• Spectroscopic Calibration: Employed as a reference material in UV-Vis, CD (Circular Dichroism), and fluorescence spectroscopy to calibrate instruments and validate experimental protocols.
• Development of Diagnostic Kits: Used in the formulation and quality control of molecular diagnostic kits that rely on specific DNA hybridization events.
• Academic & Educational Use: Provides a well-defined nucleic acid polymer for teaching advanced concepts in biochemistry and molecular genetics.

Basic Information

Product Name	Poly(D(A-C).D(G-T))
CAS No.	64816-49-5
Molecular Formula	Contact for details
Molecular Weight	Variable (Polymer)
Synonyms	Poly(dA-dC)·Poly(dG-dT); Poly(deoxyadenylic-deoxycytidylic acid)·Poly(deoxyguanylic-deoxythymidylic acid); Alternating dA-dC / dG-dT Copolymer; Poly(dA-dC):Poly(dG-dT); Poly(dA-dC).Poly(dG-dT); DNA Copolymer (dA-dC, dG-dT); Synthetic Alternating DNA Duplex
EINECS	Contact for details

Quality Control

Our Poly(D(A-C).D(G-T)) is produced and handled under stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized for critical parameters including base composition, strand integrity, and absorbance ratios. A comprehensive Certificate of Analysis (COA) detailing purity, sequence verification, and physical properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Avoid repeated freeze-thaw cycles. Solutions should be prepared using nuclease-free buffers and handled with appropriate sterile techniques to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white lyophilized solid or film
Identification (UV-Vis)	Characteristic absorbance profile with A260/A280 ratio as specified
Assay (HPLC)	≥ 95% (by area normalization)
Base Composition	Confirms 1:1 alternating dA-dC / dG-dT sequence
Molecular Weight Range	As per customer requirement (e.g., 5-20 base pairs)
Endotoxin Level	< 0.1 EU/μg (if specified for biological use)
Solubility	Soluble in TE buffer or nuclease-free water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.