Description

2'-O-Methylguanosine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog where the 2'-hydroxyl group of the ribose sugar is substituted with a methyl group. This modification confers significant nuclease resistance, making it a critical reagent for applications requiring enhanced stability of RNA molecules. It is an essential building block for researchers and manufacturers in the fields of molecular biology, diagnostics, and therapeutic oligonucleotide synthesis. The compound is supplied as a high-purity, lyophilized solid to ensure optimal performance in sensitive enzymatic and synthetic processes.

Application

• Therapeutic Oligonucleotide Synthesis: A key raw material for manufacturing antisense oligonucleotides (ASOs), siRNA, and aptamers with improved metabolic stability.
• In Vitro Transcription (IVT): Used to produce nuclease-resistant RNA transcripts for structural studies, diagnostics, and mRNA vaccine research.
• Enzymatic Labeling & Sequencing: Employed in next-generation sequencing (NGS) workflows and enzymatic labeling techniques where incorporation of a stable nucleotide is required.
• Biochemical Research: Serves as a substrate for studying RNA polymerase and reverse transcriptase enzyme kinetics and fidelity.
• Diagnostic Probe Development: Integral for creating stable, hydrolysis-resistant probes for FISH, microarrays, and other hybridization-based assays.
• X-ray Crystallography & NMR: Used to prepare modified RNA for structural biology studies to understand RNA-protein interactions and folding.

Basic Information

Product Name	2'-O-Methylguanosine 5'-Triphosphate
CAS No.	61556-44-3
Molecular Formula	C11H18N5O14P3
Molecular Weight	561.21 g/mol
Synonyms	2'-O-Methyl-GTP; 2'-OMe-GTP; 2'-O-Methylguanosine-5'-triphosphate; N2-Dimethylguanosine-5'-triphosphate (related); m2,2,7GTP (related); 2'-Methyl GTP; Guanosine, 2'-O-methyl-, 5'-(tetrahydrogen triphosphate)
EINECS	Contact for details

Quality Control

Our 2'-O-Methylguanosine 5'-Triphosphate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements for life science research and GMP-grade therapeutic applications. Certificates of Analysis (COA) detailing purity, impurity profile, and endotoxin levels are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Related Substances (HPLC)	Individual impurity ≤ 1.0%
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.