Description

Poly(Da-Idu) CAS NO 59164-41-9 is a synthetic polynucleotide, specifically a copolymer of deoxyadenylic acid (dA) and deoxyinosinic acid (dI). This compound is a critical research-grade material valued for its defined sequence and predictable base-pairing behavior. It serves as an essential tool for molecular biologists and biochemists in applications such as nucleic acid hybridization studies, polymerase chain reaction (PCR) optimization, and the development of diagnostic assays.

Application

• Nucleic Acid Hybridization Probes: Used as a model copolymer for optimizing and calibrating hybridization conditions in Southern, Northern, and dot/slot blot analyses.
• PCR and qPCR Controls: Acts as a non-target template or a synthetic standard for establishing assay sensitivity, specificity, and for generating standard curves in quantitative PCR.
• Enzyme Substrate Studies: Serves as a defined substrate for investigating the activity, kinetics, and specificity of nucleases, polymerases, and other DNA-modifying enzymes.
• Diagnostic Assay Development: Employed in the research and development phase of molecular diagnostic kits as a stable and consistent synthetic nucleic acid target.
• Biophysical Research: Utilized in studies of DNA structure, stability, and thermodynamics due to its predictable duplex-forming properties with complementary sequences.
• Teaching and Training: Provides a safe and stable synthetic DNA material for educational purposes in university and professional laboratory training courses.

Basic Information

Product Name	Poly(Da-Idu) CAS NO 59164-41-9
CAS No.	59164-41-9
Molecular Formula	(C10H12N5O6P)n • (C10H13N4O7P)n
Molecular Weight	Variable (Polymer)
Synonyms	Poly(deoxyadenylic-deoxyinosinic) acid; Poly(dA-dI); Poly(dA-dI) copolymer; Poly(dA-dI) sodium salt; Poly(dA-dI) duplex; dA-dI Copolymer; Deoxyadenylic-deoxyinosinic acid copolymer; Synthetic DNA copolymer (dA-dI)
EINECS	Contact for details

Quality Control

Our Poly(Da-Idu) is manufactured under controlled conditions to ensure batch-to-batch consistency and high purity. Each lot undergoes rigorous analytical testing, including assessment of base composition, molecular weight distribution, and absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance ratios (A260/A280), and physical state is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For extended storage of reconstituted solutions, aliquot and store at -70°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized solid or film
Identification (UV-Vis)	Characteristic absorbance maximum at ~260 nm
Purity (HPLC)	≥ 95% (Polymer peak)
Base Ratio (dA:dI)	~1:1 (by HPLC or enzymatic digestion)
A260/A280 Ratio	1.8 - 2.0
Molecular Weight	Specified range available (e.g., 500-5,000 base pairs)
Solubility	Soluble in TE buffer or nuclease-free water
Endotoxin Level	< 0.1 EU/µg (upon request for specific grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.