Description

Coelenterazine is a highly sensitive, naturally occurring luciferin that serves as the substrate for a variety of marine bioluminescent reactions. Its core value lies in enabling highly sensitive detection and quantification in biochemical assays, particularly for calcium ion (Ca²⁺) monitoring and reporter gene studies. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and life science sectors who require reliable, high-performance reagents for advanced cellular imaging and molecular diagnostics.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: A critical substrate for studying protein-protein interactions in live cells.
• Calcium Ion (Ca²⁺) Sensing: Used with aequorin and other photoproteins as a highly sensitive intracellular calcium indicator.
• Reporter Gene Systems: Enables real-time, non-invasive monitoring of gene expression and promoter activity.
• High-Throughput Screening (HTS): Applied in drug discovery platforms for target validation and compound library screening.
• Molecular Imaging: Facilitates in vivo and in vitro imaging of biological processes with low background noise.
• ATP Detection & Luciferase Assays: Serves as a co-reactant in certain luciferase-based assays for metabolic activity and cytotoxicity testing.
• Environmental Monitoring: Used in biosensors for detecting specific pollutants or toxins.
• Research Reagent: Fundamental tool for academic and industrial research in cell biology, neurobiology, and pharmacology.

Basic Information

Product Name	Coelenterazine
CAS No.	55779-48-1
Molecular Formula	C26H21N3O3
Molecular Weight	423.47 g/mol
Synonyms	Coelenterazine native; 2-Deoxycoelenterazine; 6-(4-Hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-7H-imidazo[3,2-a]pyrazin-3-one; CLZN-n; Benzyl-coelenterazine; DeepBlueC™ (trade name); CTZ
EINECS	Contact for details

Quality Control

Our Coelenterazine is produced under stringent quality management systems to ensure batch-to-batch consistency and high purity for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (e.g., argon or nitrogen), as the compound is easily oxidized and light-sensitive. Allow the product to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescence Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.